中文摘要：

为了探讨家蚕抗病毒感染的免疫应答途径,通过非转座子介导将家蚕信号转导子及转录激活因子（STAT）基因表达盒（BmSTAT）导入家蚕卵巢培养细胞（BmN）,经吉欧霉素（zeocin）筛选获得过表达BmSTAT的稳定转化BmN细胞。修饰型线性化家蚕杆状病毒Bm-BacPAK6感染结果显示：病毒感染后转化BmN细胞和正常BmN细胞的BmSTAT基因表达水平分别提高了32.70%和14.63%,表明家蚕JAK/STAT途径对杆状病毒感染有应答。比较转化BmN细胞和正常BmN细胞对杆状病毒感染的抵抗性,发现病毒对转化BmN细胞的感染比例低于正常BmN细胞,二者之间的感染率相差8.28～18.02个百分点;在感染病毒的转化BmN细胞中,β-半乳糖苷酶基因的表达水平比病毒感染正常BmN细胞的略低,推测这与转化BmN细胞中的BmSTAT水平（JAK/STAT途径信号）高于正常BmN细胞有关。然而,在BmN细胞中,BmSTAT基因的过表达尚不足以明显提高细胞对病毒感染的抵抗力。研究结果为进一步探讨家蚕JAK/STAT途径在病毒感染应答方面的作用奠定了基础。

英文摘要：

In order to investigate the immune response pathway to anti-virus in silkworm（Bombyx mori）,the expression cassette of Bombyx mori signal transducer and activator of transcription（BmSTAT） was introduced into Bombyx mori ovarian culture cells（BmN） via non-transposable element,and the stably transformed BmN cells over-expressing BmSTAT were acquired by means of zeocin screening.After being infected by modified linearized Bombyx mori baculovirus Bm-BacPAK6,the results showed that the expression levels of BmSTAT gene were enhanced by 32.70% and 14.63% in the transformed BmN cells and in the normal BmN cells respectively,indicating that the JAK/STAT pathway of silkworm did respond to the infection of baculovirus.A comparison on the resistance of transformed BmN cells and normal BmN cells against baculovirus infection showed that the ratio of infected cells was lower in the transgenic BmN cells than that in the normal BmN cells,of which difference of infected ratios between them ranged from 8.28 to 18.02 percentage point.Moreover,the expression level of β-galactosidase in infected transformed BmN cells was slightly lower than that in infected normal BmN cells,which might be related to the higher level of BmSTAT（signal of JAK/STAT pathway） in the transfor-med BmN cells than that in the normal BmN cells.However,in BmN cells,the over-expression of BmSTAT gene was not sufficient to enhance resistance of the cells against viral infection.These research results lay a good foundation for further investigations on the functions of JAK/STAT pathway in response to baculovirus infection.