中文摘要：

【目的】构建能够感染家蚕和茶尺蠖的重组家蚕核型多角体病毒（rBmNPV），解决EoNPV扩增困难而不易获得的难题。【方法】通过Bac-to-Bac系统构建具备BmNPV的多角体蛋白基因（polh）表达盒和EoNPV的解旋酶基因（hsl）表达盒的重组家蚕核型多角体病毒。【结果】家蚕或家蚕细胞扩增的rBmNPV可以感染茶尺蠖并致病，表明rBmNPV的宿主域拓展至茶尺蠖。【结论】成功构建了能够感染茶尺蠖的rBmNPV，为利用家蚕作为宿主来生产重组病毒杀虫剂打下基础。

英文摘要：

[ Objective ] The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity. [Method] Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. [Result] rBmNPV amplified from silkworm or BraN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. [Conclusion] rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.