中文摘要：

OBJECTIVE:To evaluate the effect of fermented extract of Kushen(Radix Sophorae Flavescentis) or non-fermented ESF on laryngeal neoplasms Hep2 cells.METHODS:Use 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells,and detect the mRNA and protein expression level of Bcl-2,Bax and Caspase-3 with reverse transcription polymerase chain reaction(RT-PCR) andWestern blot.RESULTS:Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm’s Hep2 cells,but and the cells did not response to the dilution 1:320 of fermented ESF,nor to the 1:1280 dilution of non-fermented ESF.As time progressed,the dilution 1:80 of fermented ESF and 1:320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression.Bax mRNA and protein were not expressed in Hep2 cells.CONCLUSIONS:Both fermented ESF and non-fermented ESF could inhibit the proliferation of Hep2 cells,and the effect of non-fermented ESF was significantly better than that of the fermented.

英文摘要：

OBJECTIVE： To evaluate the effect of fermented ex- tract of Kushen （Radix Sophorae Flavescentis） or non-fermented ESF on laryngeal neoplasms Hep2 cells. METHODS： Use 3-（4,5-dimethylthiazol-2-yl）-2,5-di- phenyltetrazolium bromide （MTT） assay to explore the effect of fermented ESF and non-fermented ESF on Hep2 cells, and detect the mRNA and protein ex- pression level of Bcl-2, Bax and Caspase-3 with re- verse transcription polymerase chain reaction （RT-PCR） and Western blot. RESULTS： Both fermented ESF and non-fermented ESF could inhibit laryngeal neoplasm＇s Hep2 cells,but and the cells did not response to the dilution 1 ： 320 of fermented ESF, nor to the 1 ： 1280 dilution of non-fermented ESF. As time progressed, the dilu- tion 1 ： 80 of fermented ESF and 1 ： 320 dilution of non-fermented ESF could significantly reduce Bcl-2 mRNA and protein expression and down-regulate Caspase-3 mRNA and protein expression. Bax mRNA and protein were not expressed in Hep2 cells. CONCLUSIONS： Both fermented ESF and non-fer- mented ESF could inhibit the proliferation of Hep2 cells, and the effect of non-fermented ESF was sig- nificantly better than that of the fermented.