中文摘要：

根据GenBank中登录的猪嵴病毒3 D基因保守序列,设计合成1对引物,以腹泻哺乳仔猪的小肠为起始材料,建立了猪峭病毒的RT-PCR检测方法,并用该方法对收集的50份哺乳仔猪腹泻样本进行检测。结果显示,该方法对模板的最小检测量是2.3pg,检测猪其他常见致腹泻病毒均为阴性,同一样品3次试验结果一致;所扩增的序列经测序分析均为猪嵴病毒的3D核苷酸序列;仔猪腹泻样本的猪嵴病毒检出率达58%。结果表明,该方法敏感度高、重复性和特异性良好,可用于猪嵴病毒的分子流行病学调查和临床病例的快速诊断。

英文摘要：

A detection method for porcine kobuvirus（PKV） with RT-PCR technique was established to detect the 3D gene of PKV from the intestinal tract of diarrhea piglets,with the detection limit about 2.3 pg RNA.50 samples of diarrhea piglets were detected by the RT-PCR method,the PKV positive rate of samples was 58%.The PCR detection method established in this study had the characteristics of sensitivity,high specificity and good repeatability,and could be used in molecular epidemiological investigation and rapid clinical diagnosis of PKV.