采用田间小区试验,以化学杀线剂(呋喃丹、涕灭威)和生物杀线剂(Snf907的发酵液、Snf0407009的发酵液)为材料,探讨杀线剂对大豆根际真菌多样性的影响。采取拉丁方设计,对大豆根际土壤定期取样、分离和鉴定,研究不同处理根际土壤真菌的动态变化。结果表明:化学杀线剂对大豆根际土壤真菌的影响较大,减少了根际土壤真菌的种类;而生物杀线剂对大豆根际真菌数量影响较小,真菌的数量和种类变化均不明显,维系了大豆根际真菌的动态平衡。在大豆成熟期根际真菌的数量比其他时期数量明显增加,可通过向生物杀线剂中添加根际真菌中的青霉菌、拟青霉菌和木霉菌来提高生物杀线剂的防治效果。
The soil of soybean filed were treated with chemical nematicides (Carbofuran, Aldicarb)and bio-nematicides ( the shaking flask liquids of Snf907 and Snf0407009 ) to study the effect of nematicide on the divisity of soil fungus. Fungus from sampling soil treated with different types of nematieide were separated and identified. Results showed that chemical nematieides reduced not only the quantity but also the divisity of rhizosphere fungi,while bio-nematicides had little effect on soil fungus and could in favor of the rhizosphere fungi balance. The populations of rhizosphere fungi at maturity of soybean were obviously higher than other growth stage. The better control effects of bio- nematieides could be realized by adding Penicillium,Paecilomyces and Trichoderma.