中文摘要：

目的：探讨12-脂氧化酶的活性代谢产物12（S）-HETE对系膜细胞和肾小球内p27^kip1表达的影响。方法：12（S）-HETE刺激的系膜细胞和皮下包埋的微型渗透泵长期持续恒速注入12（S）-HETE刺激的大鼠提取的肾小球来观察p27^kip1的表达。采用总蛋白量/细胞数的比值作为细胞肥大的评价指标,利用RT-PCR和Western blot方法检测p27^kip1mRNA和蛋白的表达。结果：12（S）-HETE刺激能够直接诱导系膜细胞发生肥大。12（S）-HETE刺激的系膜细胞内p27^kip1mRNA水平没有变化,而p27^kip1蛋白的表达明显增加。然而,在微型渗透泵持续恒速注入12（S）-HETE刺激的大鼠提取的肾小球内p27^kip1mRNA水平和蛋白的表达均增加。结论：12（S）-HETE能够通过促进p27kip1的高表达而参与细胞周期的调控,是引起肾小球系膜细胞和肾小球肥大、衰老的重要原因之一。

英文摘要：

Objective：To investigate the effect of 12（S）-HETE on the p27^kipl expression in mesangial cells and glomemli. Methods： Mesangial cells were exposed to 12（S）-HETE. 12（S）-HETE was infused to rats by osmotic mini-pump. Total protein content measurement for cell hypeilrophy,RT-PCR for mRNA expression and Westem blot for protein expression were performed respectively. Results： 12（ S）-HETE stimulation induced mesangial cell hypertrophy and 1：）27^kipl protein expression, but not p27^kipl mRNA expression. Furthermore, p27^kipl mRNA and protein expression in the glomeruli were significantly increased by 12（S）-HETE stimulation using osmotic mini-pump. Conclusion： 12（S）- HETE plays an important role in the pathogenesis of glomendar cell hypertrophy and senescence through upregulation of p27^kipl expression.