中文摘要：

目的观察氟化物对体外培养乳鼠软骨细胞超微结构的影响。方法采用细胞培养的方法，原代培养昆明乳鼠软骨细胞，传第3代后按染氟剂量不同分为0（对照）、5、10、20、40mg／L组，染氟培养10d后，制备乳鼠软骨细胞超薄切片，透射电镜下观察软骨细胞的超微结构改变。结果电镜下5、10mg／L组软骨细胞增殖，双核细胞数量增多，细胞器发达，可见囊池扩张；20、40mg／L组细胞表面微绒毛减少，部分细胞染色质固缩，并凝结成块状，聚集在核膜周边，核基质密度下降，核膜迂回曲折，胞浆内线粒体空泡化，有些细胞胞浆内可见大量的囊泡，泡内含有电子致密物质；在40mg／L组中可见到凋亡细胞。结论高氟可以引起乳鼠软骨细胞超微结构的损伤。

英文摘要：

Objective To investigate the effects of fluoride of ultra - structure of chondrocyte of culture in vitro. Meth- ods The third generation of primary cultured chondrocytes were exposed to the concentration of 0 （control） ,5,10,20,40 mg/L fluoride for 10 days. Chondrocytes of newborn mouse were collected and ultra - thin sections were made and chondrocyte ultra - styucture was observed under eletronic microscope. Results After exposed to fluoride for 10 days, the proliferation was present in the chondrocyte of 5,10 mg/L groups and shrinked chromatine apoptosed chondrocytes were seen in 20,40 mg/L groups. Some mitochondria were seen swelling, the electronic density in this site increased, microvilli of chondroeyte decreased significantly. Conclusions Higher doses of fluoride can damage the ultra - structure of chondrocyte of newborn mouse.