中文摘要：

目的 研究氟对体外培养成骨细胞中Runx2和Osterix及对其下游基因胶原（COL）I A2表达的影响.方法 体外培养人成骨细胞,按染氟（NaF）剂量将成骨细胞分为0（对照）、0.625、1.250、2.500、5.000、10.000、20.000、40.000、80.000、160.000 mg/L组,培养24 h后收集细胞,提取RNA,采用荧光定量反转录聚合酶链反应[Real-time（RT）-PCR]方法检测Runx2和Osterix mRNA及下游基因COL I A2的表达.结果 成骨细胞在体外染氟培养24 h后,当NaF剂量为0.625、1.250、2.500、5.000、10.000、20.000 mg/L时,Runx2 mRNA表达水平（388.00±41.80、209.00±25.80、42.80±4.52、63.00±16.10、24.30±4.23、16.20±4.32）均高于对照组（1.00±0.12,P均＜0.05）;当NaF剂量为40.000、80.000、160.000、mg/L时,Runx2 mRNA表达水平（0.40±0.05、1.91±0.28、4.87±1.36）与对照组比较,差异无统计学意义（P＞0.05）.当NaF剂量为1.250、2.500、5.000mg/L时,Osterix mRNA的表达水平（4.04±1.67、229.00±51.00、46.40±10.60）均高于对照组（1.00±0.42,P均＜0.05）;当NaF剂量为10.000、20.000、40.000、80.000、160.000 mg/L时,Osterix mRNA的表达水平（0.16±0.07、0.13±0.01、1.73±0.54、0.01±0.01、0.09±0.01）与对照组比较,差异无统计学意义（P均＞0.05）;当NaF剂量为0.625、1.250、2.500、5.000、10.000、20.000 mg/L时,COL I A2 mRNA的表达水平（2.27±0.89、8.03±2.31、14.20±2.75、7.66±1.34、8.96±2.30）均高于对照组（1.00±0.04,P＜0.05）;当剂量为160 mg/L时,COL I A2 mRNA的表达水平（0.54±0.01）低于对照组（P＜0.05）.结论 氟可影响成骨细胞Runx2和OsterixmRNA表达,表达水平与染氟的剂量有关.Runx2和Osterix又可以调节COLI A2 mRNA表达.

英文摘要：

Objective To study the effect of fluoride on expression of osteoblast Runx2, Osterix and their downstream COL I A2 in vitro. Methods Human osteoblast Saos-2 was cultured in vitro. The cells were grouped according to fluoride（NaF） dose used： 0（control ）, 0.625,1.250,2.500,5.000,10.000,20.000,40.000,80.000,160.000 mg/L. Cells were collected after 24 h culture, RNA extracted, and the mRNA expression of Runx2 and Osterix and downstream genes COL I A2 was detected using fluorescent quantitative reverse transcription polymerase chain reaction [Real-time （RT）-PCR]）. Results After 24 h in vitro cell cultivation with NaF, the expression of Runx2 in 0.625,1.250,2.500,5.000,10.000,20.000 mg/L groups（388.00 ± 41.80,209.00 ± 25.80,42.80 ±4.52,63.00 ± 16.10,24.30 ± 4.23,16.20 ± 4.32） was higher than that of the control group（ 1.00 ± 0.12, all P 〈0.05）. The expression of Runx2 in 40.000,80.000,160.000 mg/L groups（0.40 ± 0.05,1.91 ± 0.28,4.87±1.36）compared with that of control group, the difference was statistically insignificant（all P 〉 0.05）.The expression of Osterix mRNA in 1.250,2.500,5.000 mg/L groups（4.04 ± 1.67,229.00 ± 51.00,46.40 ± 10.60） was higher than that of the control group（ 1.00 ± 0.42,all P 〈 0.05）. The expression of Osterix mRNA in 10.000,20.000,40.000,80.000,160.000 mg/L groups（0. 16 ± 0.07,0.13 ± 0.01,1.73 ± 0.54,0.01 ± 0.01, 0.09 ± 0.01） compared with that of control group, the difference was statistically insignificant （all P 〉 0.05）. The expression of COL I A2 mRNA in 0.625,1.250,2.500,5.000,10.000,20.000 mg/L groups （2.27 ± 0.89,8.03 ± 2.31,14.20 ± 2.75,7.66 ± 1.34,8.96 ±2.30） was higher than that of the control group （1.00 ± 0.04, all P 〈 0.05）. The expression of COL I A2 mRNA in 160.000 mg/L（0.54 ± 0.01 ） was lower than that of the control group（P 〈 0.05）. Conclusions Fluoride may affect mRNA expression of Osterix and Runx2 in osteoblast and their expression level is related to fluoride concentration.Ru