中文摘要：

目的：探讨氟对体外培养软骨细胞中COLⅨA3基因mRNA的表达影响.方法：采用SYBR Green Ⅰ嵌合荧光法进行实时定量PCR的方法,检测不同剂量氟对体外培养软骨细胞中COLⅨA3基因mRNA的表达影响,染氟剂量分别为0、5、10、20、40 mg/L,染氟10d.结果：实时定量PCR检测显示各组均可检测到COLⅨA3 mRNA,相对定量比由对照组到高剂量组约为：100：134：200：104：129,染氟实验组中5mg/L、10mg/L组表达量高于对照组和40mg/L组,且以10mg/L组表达量最高,是对照组的2倍.随着染氟剂量增加,软骨细胞COLⅨA3基因mRNA表达量降低.结论：不同剂量氟对软骨细胞中COLⅨA3基因mRNA表达的影响不同,低剂量氟可以促进COLⅨA3基因mRNA的表达,随剂量增加氟的促进作用减弱.

英文摘要：

Objective： To detect the influence of fluoride on COLⅨA3 gene expression of Chondrocyte. Methods： The influence of different doses of fluorine on the mRNA expression of COL ⅨA3 of chondrocyte cultured in vitro was investigated by SYBR Green I methods. Chondrocytes were exposed to fluorine as 0（the control）, 5, 10, 20, 40mg/L groups respectively for ten days. Results： COL Ⅸ A3 mRNA can be detected in each group by real time RT-PCR. The relative ration rate from control group to higher-dose groups was as following： 100：134：200：104：129; 5, 10mg/1 of experiment groups was higher than control group and 40mg/l group, the expression of 10mg/1 group was the highest. With the increase of dose, the expression of genes decreased. Conclusions： The influence on COLⅨA3 expression in cartilage cells varies with the dose of fluorine, the fluorine of low doses can promote the expression of COL Ⅸ A3, with the increasing of dose, its promotion will decrease.