中文摘要：

目的 构建NT4-p53（N15）-Ant融合基因表达盒并进行序列分析。方法应用互为模板的引物PCR技术及T载体克隆法克隆p53（N15）-Ant基因，筛选阳性克隆、酶切鉴定并测序。扩增阳性重组质粒后限制性内切酶切取p53（N15）-Ant片段连入pBV220／NT4质粒。结果 克隆了p53（N15）-Ant基因，经酶切及测序证实结果正确；重组质粒pBV220／NT4p53（N15）Ant经限制性内切酶及琼脂糖凝胶电泳，结果 显示酶切片段大小和理论值一致。结论 通过分子克隆体外重组技术成功制备了含有NT4-p53（N15）-Ant表达盒的pBV220质粒，为进一步开展肿瘤的基因治疗奠定了基础。

英文摘要：

Objective To construct the expression box of fusion gene NT4-p53（N15）-Ant. Methods The gene of p53 （N15）-Ant was obtained by PCR of two primers templating with each other and T-vector cloning method. The positive clone was identified and analyzed by the restriction enzymes and sequencing respectively. After digested with restriction enzyme, the interest gene of p53（N15）-Ant was subcloned into the plasmid pBV220/ NT4. Results The gene of p53（N15）-Ant was confirmed by the digestion of restriction enzyme and sequencing. The recombinant plasmid pBV220/NT4p53 （N15）Ant was identified by the digestion of restriction enzyme and agarose gel electrophoresis and the results conformed theoretical values. Conclusion The plasmid pBV220 containing the expression box of NT4-p53 （N15）-Ant was successfully constructed by molecular cloning and recombination techniques in vitro, which will guide further study on gene therapy of cancer.