中文摘要：

力生长因子（MGF,mechanoGgrowthfacGtor）是由Yang等发现并命名,是细胞对力刺激响应的产物,对人骨髓间充质干细胞（hMSC,humanmesenGchymalstemcells）有重要的调控作用.利用静电纺丝技术（electrospinning）及相分离法制备了基于聚已内酯（PCL,polycaprolactone）的含MGF 的葡聚糖纳米微球的纤维支架,间接性地保护了MGF活性,采用扫描电镜和透射电镜对微球和纤维支架进行了基础表征.同时,进一步采用激光共聚焦显微镜和酶标仪分别考察了纳米微球中释放出来的MGF 对细胞的作用.研究结果表明含MGF的纳米微球支架能够显著促进hMSC增殖和迁移.此静电纺丝纤维支架能够较好地保护MGF的活性,有望在组织工程领域得到应用.

英文摘要：

Mechano-growth factor (MGF)was found and named by Yang,generated by cells in response to me-chanical stimulation,playing a key role in regulating mesenchymal stem cell (MSC)function.In this study, pre-formulated dextran glassy nanoparticles (DGNs)loaded with MGF through freezing-induced phase separa-tion were electrospun into a polycaprolactone (PCL)copolymer fiber to secure the bioactivity of MGF,which was characterized by field emission scanning electron microscopy (FESEM)and transmission electron microsco-py (TEM),and then bioactivity retention was certificated by the change of hMSC behaviour with confocal laser scanning microscopy and spectrophotometric microplate reader.The enhanced cell proliferation and migration show that the MGF-DGNs-loaded PCL fibrous membrane can release MGF sustainably and secure the bioactivi-ty of MGF better than other PCL fibrous membranes,which may help find MGF-based fibrous membranes ap-plication of MSC in tissue engineering.