中文摘要：

目的：了解Apo E基因敲除对淋巴细胞的影响。方法用不同抗体标记Apo E基因敲除及同窝野生对照小鼠的外周血、脾脏、骨髓等细胞，应用流式细胞仪进行分析，比较两组之间的差异。结果对检测结果进行统计分析发现，与野生型小鼠相比，Apo E基因敲除小鼠的外周血、脾脏淋巴细胞、短期造血干细胞均有改变，骨髓前体B淋巴细胞、胸腺T淋巴细胞、长期造血干细胞等没有显著变化。结论 Apo E基因敲除后，小鼠外周血及脾脏B淋巴细胞减少，骨髓短期造血干细胞增加，但B及T淋巴细胞发育，以及骨髓长期造血干细胞、淋系共同祖细胞都没有显著变化。

英文摘要：

Objective To study the influence of Apo E gene knockout on the lymphocytes . Methods Cells from the peripheral blood, spleen and bone marrow of Apo E knockout and wildtype mice were stained with kinds of antibodies , and analyzed by flow cytometry .Results Compared with wildtype mice , significant differences were found in B and T lymphoctes in the peripheral blood and spleen , but there was no significant difference in pre B cells , T lymphocytes in the thymus and long term hematopoietic stem cell in the Apo E knockout mice .Conclusion Numbers of B lymphocytes decreased in the peripheral blood and spleen , but there was no significant difference in B , T lymphocytes development , and numbers of long term hematopoietic stem cell in Apo E gene knockout mice .