中文摘要：

【目的】研究丁香酸（syringic acid,SY）对舞毒蛾核型多角体病毒（LdNPV）致死及亚致死作用的影响,为舞毒蛾Lymantria dispar L.的防治提供科学依据。【方法】采用食料给毒法进行生物测定,评估LdNPV及SY与LdNPV共同对舞毒蛾2龄幼虫的致死和亚致死作用。【结果】取食添加丁香酸人工饲料和正常人工饲料的舞毒蛾幼虫LdNPV致死中浓度（LC50）分别为91.1 OBs/μL和194.8 OBs/μL。当LdNPV浓度为2 400 OBs/μL,取食添加SY的饲料及正常人工饲料的舞毒蛾幼虫LdNPV致死中时间（LT_（50））分别为9.3 d和10.1 d,当LdNPV浓度为24 000 OBs/μL时,其LT50分别为7.8 d和8.1 d,LdNPV的浓度越高致死中时间越短。丁香酸增加了LdNPV对舞毒蛾幼虫的致病力,降低了LdNPV对舞毒蛾幼虫的致死中浓度,缩短了致死中时间。LdNPV对取食正常饲料的雌、雄幼虫历期和蛹期及雌蛾寿命影响不显著,但显著缩短了雄虫寿命,降低了平均每雌产卵量。LdNPV对取食添加丁香酸饲料的幼虫产生明显的亚致死效应,并且LdNPV浓度高于240OBs/μL时雌、雄幼虫历期延长、雄蛾寿命缩短,雄性羽化率降低,雌成虫产卵量降低,产卵量较对照减少一半左右。【结论】结果提示,丁香酸可提高LdNPV对舞毒蛾幼虫的致病力,增加LdNPV对舞毒蛾的亚致死作用。

英文摘要：

【Aim】 This study aims to evaluate the effects of syringic acid on the lethal and sublethal effects of Lymantria dispar nucleopolyhedrovirus （LdNPV） against larvae of the gypsy moth, L. dispar, so as to provide a scientific basis for the prevention and control of this insect pest. 【Methods】 Diet plus was used to assess the lethal and sublethal effects of LdNPV with and without syringic acid on the 2nd instar larvae of L. dispar. 【Results】 The median lethal concentration （LC50） values of LdNPV to the 2nd instar larvae of L. dispar fed on the artificial diets with and without syringic acid were 91.1 OBs/μL and 194.8 OBs/μL, respectively. The median lethal time （LT50） values of LdNPV at the concentration of 2 400 OBs/μL to the 2nd instar larvae fed on the artificial diets with and without syringic acid were 9.3 and 10.1 d, respectively. The median lethal time （LT50） values of LdNPV at the concentration of 24 000 OBs/μL to the 2nd instar larvae fed on the artificial diets with and without syringic acid were 7.8 and 8.1 d, respectively. The higher the concentration of LdNPV, the lower the LT50 value. Syringic acid added in the artificial diet enhanced the lethal effects of LdNPV against L. dispar larvae, and caused the lower LC50 and LT50 values of LdNPV against L. dispar larvae as compared with the diet without syringic acid. When the larvae were fed the artificial diet, the sublethal effects of LdNPV on larval duration, pupal duration and female adult longevity were not significant; when the LdNPV concentration was higher than 240 OBs/μL, however, the male adult longevity was shortened and the number of eggs laid per female was less than those of the control. The sublethal effects of LdNPV on L. dispar larvae fed on the artificial diet with syringic acid and high concentration of LdNPV were significant： the female and male larval duration was prolonged, the male adult longevity was shortened, and the male adult emergence rate and the number of eggs laid per female reduced. 【Concl