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外源茉莉酸诱导的青杨叶片保护性酶活性变化及其对舞毒蛾幼虫生长发育的影响
  • ISSN号:0454-6296
  • 期刊名称:昆虫学报
  • 时间:2013.3.20
  • 页码:270-275
  • 分类:Q965.9[生物学—昆虫学]
  • 作者机构:[1]内蒙古农业大学林学院,呼和浩特010019, [2]内蒙古农业大学农学院,呼和浩特010019, [3]乌兰察布市森林病虫防治检疫站,内蒙古乌兰察布012000
  • 相关基金:国家自然科学基金项目(31160161)
  • 相关项目:外源茉莉酸诱导杨树抗性对舞毒蛾LdNPV发生的影响及机制
中文摘要:

为探讨外源茉莉酸对青杨Populuscathayana的诱导抗性及其对舞毒蛾Lymantriadispar的影响,室内对青杨扦插苗喷施不同浓度的茉莉酸(对照为0.17%丙酮),分别在喷施后1,5和10d采集叶片分析其保护性酶活性的变化,并接舞毒蛾幼虫于青杨苗木上观测其长发育情况。结果表明:0.1和0.001mmol/L两种浓度的茉莉酸(JA)处理均使青杨叶过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、胰凝乳蛋白酶抑制剂(cI)和胰蛋白酶抑制剂(TI)活性较对照增加(P〈0.05)。取食茉莉酸诱导的青杨苗木后,舞毒蛾幼虫的发育历期延长,体重降低。0.1mmol/L茉莉酸诱导的青杨苗木5d后接虫,使舞毒蛾幼虫的发育历期显著延长,较对照长8d;接虫21d后称重时,取食茉莉酸诱导的青杨叶片的幼虫体重较对照组降低了50%~100%,该结果说明外源茉莉酸诱导青杨产生了对舞毒蛾明显的抗虫性。

英文摘要:

In order to investigate the resistance induced by exogenous jasmonic acid in Populus cathayana and the effects on development of the gypsy moth, Lymantria dispar, two concentrations of jasmonic acid solution (0. 1 and 0. 001 mmol/L) were sprayed onto green poplar seedlings while the control was sprayed with 0.17% acetone, the change in activities of protective enzymes in treated leaves was tested at 1,5, and 10 d post JA treatment, and the developmental duration and body weight of L. them were measured. The results showed that the activities of peroxidase (POD) , polyphenol oxidase ( PPO), phenylalanine ammonia-lyase ( PAL), ehymotrypsin inhibitor (CI) and trypsin inhibitor (TI) in leaves treated with two concentrations of jasmonic acid were significantly higher than that of the control (P 〈 0.05 ). The developmental duration of L. dispar larvae fed on JA-induced green poplar seedlings was prolonged, while their body weight was reduced. The developmental duration of L. dispar larvae fed on 0.1 mmol/L JA-induced seedlings was 8 d longer than that of the control. The larval body weight of L. dispar measured at 21 d post JA treatment was decreased by 50% - 100%. The results suggest that exogenous jasmonic acid could induce resistance in green poplar to L. dispar larvae.

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期刊信息
  • 《昆虫学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国昆虫学会 中国科学院动物研究所
  • 主编:黄大卫
  • 地址:北京东中关村中国科学院动物研究所
  • 邮编:100101
  • 邮箱:kcxb@ioz.ac.cn
  • 电话:010-64807173 010-64807099
  • 国际标准刊号:ISSN:0454-6296
  • 国内统一刊号:ISSN:11-1832/Q
  • 邮发代号:2-153
  • 获奖情况:
  • 国家期刊方阵“双百期刊”
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:16190