中文摘要：

【目的】为探讨外源茉莉酸（jasmonic acid,JA）诱导的青杨Populus cathayana Rehd.抗性对舞毒蛾Lymantria dispar（L.）幼虫食物利用的影响。【方法】在室内对青杨苗木喷施0.001和0.1 mmol/L两种浓度的茉莉酸,对照喷0.2%的丙酮水溶液,喷施后1,5和10 d分别采集叶片,分析其初生和次生物质含量的变化。另选喷施0.1 mmol/L茉莉酸的青杨苗木,喷施后1,5和10 d分别接舞毒蛾2龄幼虫,单头饲养,测定其取食量、体重和排粪量及发育历期,统计分析其食物消化率、转化率和利用率。【结果】两种浓度茉莉酸处理均使青杨叶片中的蛋白质和可溶性糖含量降低,而木质素、单宁、黄酮和酚类物质含量增加。蛋白质和可溶性糖在处理第10天时含量最低,其中高浓度茉莉酸处理较对照分别降低46.5%和49.1%,低浓度处理分别降低30.6%和22.8%。叶片中酚类物质含量在高浓度处理第10天时增幅最大,较对照增加102%。木质素、黄酮和单宁酸在处理第5天时的含量最高,其中高浓度处理分别较对照增加113%,75%和57%。用0.1 mmol/L茉莉酸诱导处理后,舞毒蛾2龄幼虫对叶片的消化率、转化率和利用率均有所降低,取食处理后1,5和10 d的青杨叶片的食物利用率较对照分别降低了29.4%,27.6%和28.2%,且幼虫体重降低、发育历期延长。【结论】结果提示外源茉莉酸诱导青杨可对舞毒蛾产生明显的生化抗性,实践中可利用这种生化抗性防治舞毒蛾的危害。

英文摘要：

【Aim】This study aims to make clear whether the exogenous jasmonic acid（ JA）-induced resistance in green poplar,Populus cathayana Rehd.,influences food utilization of larvae of the gypsy moth,Lymantria dispar（ L.）. 【Methods】JA solution at the concentrations of 0. 001 mmol / L and 0. 1mmol / L（ diluted with 99. 5% acetone first and then with distilled water） was sprayed onto green poplar seedlings,while the control was sprayed with the 0. 2% acetone solution. The changes in the contents of primary and secondary metabolites,mainly proteins,soluble sugars,phenols,lignins,flavones and tannins,were tested on the 1st,5th and 10 th day post treatment. To assess the food utilization of L.dispar larvae,other green poplar seedlings sprayed with 0. 1 mmol / L JA solution were used to rear the newly molted 2nd instar larvae,and the body weight,food consumption,feces and the duration of the2 nd instar larvae were measured. 【Results】Compared to the control,the contents of proteins and soluble sugars in green poplar seedlings sprayed with exogenous JA solution decreased,but the contents of phenols,lignins,flavones and tannins increased significantly. The contents of soluble sugars and proteins in green poplar leaves decreased to the lowest level on the 10 th day post JA treatment,and reduced by 46. 5%and 49. 1% in the high-concentration JA treatment group,and 30. 6% and 22. 8% in the the lowconcentration JA treatment group,respectively,compared to the control. The total content of phenols in green poplar leaves was at the highest level on the 10 th day post JA treatment and increased by 102% in the high-concentration JA treatment group compared to the control. The contents of lignins,tannins and flavones in green poplar leaves reached a peak on the 5th day post JA treatment and increased by 113%,75% and 57% compared to the control,respectively. The approximate digestibility,conversion rate,utilization rate of food for L. dispar larvae fed on the JA-induced green poplar leaves all decreased compared with t