中文摘要：

目的探讨视网膜母细胞瘤Rb94基因与放射联合治疗后裸鼠食管癌细胞中Rb蛋白和Ki67蛋白的表达情况。方法将食管癌K150细胞接种裸鼠建立肿瘤模型,实验分为5组,对照组未进行处理,Ad-Lac Z组为含Lac Z基因但不含Rb94基因的对照腺病毒,分别于处理后即刻、处理后的第3、7天以Ad-Lac Z感染瘤体,Ad-Rb94组分别于处理后即刻、处理后的第3、7天以Ad-Rb94感染瘤体,照射组分别于第1、4、8天进行4 Gyγ射线局部照射瘤体,联合组在Ad-Rb94感染后进行4 Gy局部照射。采用免疫组化方法观察肿瘤组织Rb蛋白和Ki67蛋白的变化,采用TUNEL法检测细胞凋亡。结果 Ad-Rb94组[（28.96±3.35）%]和联合组[（33.13±1.59）%]Rb蛋白阳性表达增加,明显高于对照组,差异有统计学意义（q=9.96、13.35,P〈0.01）;Ad-Rb94组和联合组Rb蛋白表达均高于照射组,差异有统计学意义（q=12.72、15.36,P〈0.01）。Ad-Rb94组[（29.15±1.65）%]、照射组[（38.16±2.53）%]和联合组[（25.18±3.06）%]Ki67蛋白阳性表达减少,与对照组比较差异有统计学意义（q=6.36、5.59、7.52,P〈0.01）;联合组Ki67蛋白表达显著低于照射组（q=6.49,P〈0.01）。AdRb94组[（14.1±3.2）%]、照射组[（17.2±3.0）%]和联合组[（24.3±3.2）%]细胞凋亡率显著增加,与对照组比较差异有统计学意义（q=6.62、7.29、10.54,P〈0.01）;联合组细胞凋亡率明显高于照射组和Ad-Rb94组,差异有统计学意义（q=6.36、7.89,P〈0.01）。结论 Rb94基因与放射联合应用协同抑瘤的机制之一是通过增强Rb蛋白表达和抑制抗凋亡蛋白Ki67表达。

英文摘要：

Objective To reveal the effects of retinoblastoma 94 gene therapy combined with radiotherapy on the expression of Rb protein and Ki67 protein in esophageal carcinoma cells of tumor- bearing nude mice. Methods The model of tumor- bearing nude mice was established by K150 cells inoculation. The cohorts were divided into five groups,blank control（ no treatment）,Ad- Lac Z group（ control adenovirus including Lac Z gene but not Rb94 gene,Ad- Lac Z was transfered into tumor xenograft on 0d,3d,7d therapy separately）,Ad- Rb94 group（ tumor xenograft was transfected with Ad- Rb94 on 0d,3d,7d therapy separately）,radiation group（ tumor xenograft was irradiated with 4Gy γ- radiation on 1d,4d,8d therapy separately） and Ad- Rb94 combined with radiation group（ combining group for short,tumor xenograft was irradiated with 4Gy after transfected with Ad- Rb94）. The expression of Rb protein and Ki67 protein in esophageal carcinoma was detected by immunohistochemical analysis. Cell apoptosis was examined by TUNEL assay.Results The expression of Rb protein in Ad- Rb94 group（28.96±3.35%） and combining group（33.13 ±1.59%）was significantly increased compared with that in control group（ q=9. 96 and 13. 5,P〈0.05）. The expression of Rb protein in Ad- Rb94 group and combining group was significantly higher than that in radiation group（q=12.72 and15.36,P〈0.01）. The expression of Ki67 protein in Ad- Rb94 group（29.15±1.65%）,radiation group（ 38.16±2.53%） and combining group（25.18±3.06%） was significantly reduced compared with that in control group（q=6.36,5.59 and7.52,respectively,P〈0.01）. The expression of Ki67 protein in combining group was significantly less than that in radiation group（q=6.9,P〈0.01）. The ratios of cell apoptosis in Ad- Rb94 group（14.1 ± 3.2%）,radiation group（17.2±3 0%） and combining group（24.3±3.2%） were significantly increased compared with that in control group（q=6.62,7.29 and 10. 54,respectively,P〈0.01）. The ratio of cell apoptosis in