中文摘要：

目的:探讨外源性Rb94基因联合放疗对卵巢癌细胞生长的抑制作用,为肿瘤的Rb94基因-放射治疗提供实验依据。方法:应用携带Rb94基因的复制缺陷型腺病毒(Ad-Rb94)于体外转染卵巢癌细胞株ES-2,用MTT比色法和流式细胞术观察Rb94基因联合放疗后ES-2细胞的生长曲线和细胞周期的变化。结果:Ad-Rb94基因组、单纯放疗组和Ad-Rb94基因联合放疗组ES-2细胞生长均明显受到抑制,与Ad-lacZ组和空白对照组比较均有显著性差异(P<0.05)。其中联合放疗组ES-2细胞生长最为缓慢,与Ad-Rb94基因组和单纯放疗组比较均有显著性差异(P<0.05)。Ad-Rb94组和放疗组大量的ES-2细胞停留在G1期,而G2期和S期细胞明显减少,联合治疗组细胞停留在G1期最多。结论:腺病毒介导的外源性Rb94基因可明显抑制ES-2细胞的生长,与放射治疗联合应用具有相加和协同效应。

英文摘要：

Objective:To study the combined effect of foreign retinoblastoma 94 gene and ionizing radiation on the growth of ovarian carcinoma cells, and explore a gene therapy approach for ovarian cancer. Methods:Rb94 gene was inserted into a replication-deficient adenovirus vector , and ovarian carcinoma cell line was infected with adenovirus-Rb94 and irradiated with γ rays. The cell growth curve and cell cycle of ES-2 cell was detected by MTT assay and flow cytometry respectively. Results:The growth of ES-2 cells tr...