中文摘要：

目的探讨重组腺病毒Ad-Rb94基因联合放射治疗对人肝癌细胞的联合抑瘤作用。方法将重组腺病毒Ad-Rb94基因导入人肝癌细胞株HepG2,观察联合放射治疗对细胞生长、细胞周期及凋亡的影响。结果Ad-Rb94基因组、放疗组和Ad-Rb94基因联合放疗组HepG2细胞的生长均受到抑制，尤其在转染后96h细胞存活数量最低，与Ad-lacZ组和空白对照组比较差异均有显著性（P〈0．05）。联合治疗组HepG2细胞生长最为缓慢，Rb94基因转染后96hAd—Rb94联合放疗组的抑制作用均明显高于Ad—Rb94组和放疗组（P〈0．05）。Ad—Rb94组、放疗组及Ad—Rb94联合放疗组HepG2停留在G2／M期的细胞增加，G0／G1期和S期细胞数量减少，凋亡细胞数量增加，其中以联合治疗组G2／M期细胞和凋亡细胞所占比例最高。结论Ad～Rb94基因联合放疗具有协同作用，有效地抑制肝癌细胞的生长．

英文摘要：

Objective To evaluate the effects of recombinant adenovirus - Rb94 and radiotherapy on the growth of human hepatic carcinoma cells. Methods Human hepatic carcinoma cells HepG2 were treated with Ad - Rb94, radiotherapy or Ad - Rb94 combined with radiotherapy respectively. The cell growth curve , cell cycle and cell apoptosis of HepG2 cells were examined. Rusults The growth of HepG2 cells transfected with Ad - Rb94, radiation and combination of Ad - Rb94 and radiation was significantly slower than that of Ad - lacZ and blank control （P 〈 0.05 ）, especially at 96h after transfection. The combination of Ad - Rb94 and radiation resulted in greater inhibition of cell growth compared with Ad - Rb94 infection and radiation at 96h after transfection（P 〈 0.05 ）. The number of cells of G2/M phases transfected with Ad - Rb94 and radiation increased, while the number cells of G0/G1 and S phases decreased; cells of apoptosis increased. The increase of cells of G2/M phases and apoptosis for the combination of Ad - Rb94 and radiation were the highest . Conclusion Ad - Rb94 gene therapy combined with radiotherapy show synergism for the inhibition of HepG2 cells growth.