中文摘要：

目的 克隆大鼠胶质细胞源性神经营养因子（GDNF）基因，构建含有GDNF基因的重组腺病毒载体，为转染神经干细胞和基因治疗奠定基础。方法 从大鼠脑组织中提取总RNA，用逆转录聚合酶链反应方法扩增出约660bp的片段，并将该片段克隆到载体pAdTrack-CMV中，进行酶切鉴定和序列分析。结果 证实成功构建了含有GDNF基因的重组腺病毒质粒。结论 克隆到正确的大鼠GDNF基因，并构建出重组质粒pad—GDNF，为下一步重组腺病毒颗粒和基因治疗打下基础。

英文摘要：

Objective To clone rat glialeellline derived neutrophie factor gone （GDNF） and construct recombinant adenovirus vector containing GDNF gent, which was then transfected into neural stem cells. Methods talRNA was extracted from rat brain tissue. A660bp positive eDNA was obtained by RT-PCR and then was cloned into the shuttle plasmid pAdTract-CMV. The cloned fragment, was sequenced and identified by restriction enzyme. Results Recombinant adenovirus plasmid containing GDFN gene was obtained successfully. Conclusion A recombinant adcnovirus plasmid containing GDNF is coustruetcd suecessfuUy which lays the foundation for further study on aene theraDv and recombinant adenovirus olasmid.