中文摘要：

目的探讨神经干细胞转染的新方法，并观察转染后目的基因的表达情况。方法原代培养神经千细胞，运用jet PEI转染试剂将目的基因胶质细胞源性神经营养因子（GDNF）和内皮素B受体基因（EDNRB基因）共转染至神经干细胞内，免疫荧光显微镜观察、流式细胞仪检测绿色荧光蛋白（GFP）表达情况，测定转染效率，RT-PCR检测目的基因表迭情况。结果成功培养出可用于基因转染的神经干细胞，转染后24h即可在免疫荧光显微镜下观察到GFP表达，流式细胞仪显示24、48和72h转染效率分别为15．36％、24．67％和25．73％。RT—PCR显示目的基因在神经干细胞内成功表达。结论运用jet PEI成功将目的基因转染至神经干细胞内，为相关神经变性疾病的基因治疗打下了实验基础。

英文摘要：

[Objectives] To investigate the optimizing method of neural stem eel/（NSC） transfection and the expression of extrinsic genes in neural stem cells. [Methods] Extrinsie genes GDNF and EDNRB were eotransfeeted into primary Cultured neural stem ceils by using jet PEI. The expression of green fluorescent protein （GFP） was measured with fluorescence microscope and flow eytometer. To detect the expression of mRNA of GDNF and EDNRB by RT-PCR. [Results] Bright green fluorescence of the transfected eells could be observed under fluorescence mieroscope after 24 h of transfeetion. Flow cytometer analysis showed that the effieieney of eotransfeetion were 15.36%, 24.67%, 25.73% in 24, 48 and 72 hours respectively. Meanwhile the expression of GDNF and EDNRB genes were detected in neural stem ceils by RT-PCR. [Conclusions] The target genes were successfully eotransfeeted into neural stem cells by using jet PEI. It provides a feasible technological platform for the polygene therapy of neural degenerative diseases.