中文摘要：

目的该文以THP-1源性巨噬细胞为研究对象,研究牙龈卟啉单胞菌脂多糖（P.g-LPS）对巨噬细胞内胆固醇代谢关键分子CD36、LOX-1表达的影响。方法以160 nmol/L佛波酯（PMA）诱导THP-1单核细胞48 h,使其分化为巨噬细胞。建立P.g-LPS与THP-1源性巨噬细胞共孵育的体外模型,在负荷50μg/m L低密度脂蛋白（LDL）的条件下,分别用浓度为0.1、1.0、10.0μg/m L P.g-LPS与巨噬细胞共孵育48 h;1μg/m L P.g-LPS与巨噬细胞共孵育24、48和72 h。采用实时荧光定量PCR和Western blot方法探讨巨噬细胞胆固醇代谢关键分子CD36和LOX-1表达变化。结果与空白对照组相比,随着P.g-LPS浓度的增加和1μg/m L P.g-LPS孵育时间的延长,巨噬细胞内CD36 mRNA和蛋白表达均逐渐下降;LOX-1mRNA和蛋白表达无明显变化。结论 P.g-LPS可促进巨噬细胞CD36的表达,对LOX-1表达无影响,从而可能对巨噬细胞脂质代谢产生影响。

英文摘要：

Objective To explore the effect of porphyromonas gingivalis lipopolysaccharides（ P. g-LPS） on the expression of CD36 and LOX-1 macrophages derived from THP-1. Methods THP-1 cells were cultured in the presence of 160 nmol / L phorbol 12-myristate 13-acetate（ PMA） for 48 h. An co-cultured in vitro model of P. g-LPS and THP-1 macrophage cells was constructed. In the presence of LDL（ 50 μg / ml）,macrophage cells were incubated with P. g-LPS（ 0. 1,1,10 μg / ml） for 48 h or with 1μg / ml P. g-LPS for24,48,72 h. The expression of CD36,LOX-1 at mRNA and protein levels was determined by real time PCR and Western blot. Results THP-1 cells were differentiated into macrophages after the addition of PMA for 48 h. P. g-LPS down-regulated the mRNA and protein expression of CD36 as concentration increased and time extended in the presence of LDL（ P〈0. 05）. However,P. g-LPS had no effect on the expression of LOX-1 at mRNA and protein levels compared with control group. Conclusions P. g-LPS down-regulated CD36,but had no effect on the expression of LOX-1,which may contribute to the excessive accumulation of cholesteryl esters and conversion to foam cells in macrophages.