中文摘要：

目的 以THP-1来源的巨噬细胞为研究对象,初步研究牙龈卟啉单胞菌（Porphyromonas gingivalis,P.g）感染促进巨噬细胞形成泡沫细胞的影响.方法 以160 nmol/L佛波酯（phorbol 12-myristate 13-acetate,PMA）诱导THP-1单核细胞48 h,使其分化为巨噬细胞.在负荷50 μg/mL低密度脂蛋白的条件下,建立P.g感染巨噬细胞的体外模型,采用胆固醇测定法检测细胞内胆固醇酯含量的变化.结果 经160 nmol/L PMA诱导48 h后,THP-1单核细胞成为巨噬细胞.6h时P.g感染组细胞胆固醇酯（CE）与总胆固醇（TC）比值（CE/TC）为0.445±0.386,24 h时,P.g感染组CE/TC值为0.557±0.430,均显著高于空白对照组和6h组（P＜0.05）.结论 本研究成功建立了P.g感染巨噬细胞的体外模型,P.g感染可显著促进巨噬细胞总胆固醇和胆固醇酯含量增加,从而可能参与心血管疾病（cardiovascular diseases,CVD）发生和发展.

英文摘要：

Objective To investigate the effect of porphyromonas gingivalis （P. g） infection on the formation of foam cell derived from THP-l-derived macrophage. Methods In order to induce macrophage differentiation,THP-1 cells were cultured in the presence of 160 nmol/L PMA for 48 h. And the model of the infection of THP-l-derived macrophage by P. g was established in vitro in the presence of LDL （50 p,g/mL）. The amounts of total cholesterol （TC） and cholesterol ester （CE） were quantified using zymochemistry method at 0,6,and 24 h,respectively. Results After the addition of 160 nmol/L phorbol 12-myristate 13-acetate （PMA） for 48 h,THP-1 cells were differentiated into maerophages for further studies. Incubation of LDL-treated macrophages with P. g caused an increase in CE and TC accumulation. Compared with control group,the P. g infection group had significantly increased the ratio of CE to TC （0. 445 ± 0.386） （P〈0.05）.Compared with Oh and6h,P, ginfection group had higher ratio （0.557 ±0.430） ofCE toTC （P〈0.05） at 24 b. Conclusions Macropbages can be formed with stimulation PMA to THP-1 monocytes after 48 h; P. g infection can promote macro- phage-derived foam cell formation,which highlights a potential role for P. g in the pathogenesis of cardiovascular diseases （ CVD）.