欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
叶酸受体在实体瘤组织细胞表面的过度表达使得叶酸介导的靶向释药系统成为治疗癌症的研究热点;纳米粒子能够逃避网状巨噬细胞（RES）的捕获并加强渗透和滞留效应（EPR）是其应用于药物控释系统的主要原因。以聚乳酸、氨基封端的聚乙二醇和叶酸为原料,采用活性酯的方法合成了聚乳酸-聚乙二醇-叶酸偶合物,并以此为载体,采用溶液挥发自组装的方法制备具有主动靶向性的纳米微粒。采用1 HNMR,对材料结构进行表征;采用荧光探针法对微粒的稳定性进行检测;采用人乳腺癌细胞（MCF-7）和成纤维细胞（CCL-110）对微粒的细胞靶向选择性进行实验。结果表明,在成功合成材料的基础上,制备的纳米粒子具有很好的细胞选择性,和同类材料相比具有较好的稀释稳定性,有望成为叶酸受体介导的靶向药物控释系统的载体材料。
英文摘要：
Folate has been employed as a targeting moiety of various anticancer agents to increase their cellular uptake within target cells since folate receptors were vastly overexpressed in several human tumors. In this study, dodecanoled-poly（D, L-lactic acid）-b-poly （ethylene glycol）-folate （Dol-PLA-PEG-FA） was synthesized from dodecanoled-poly（D, L-lactic acid）, H2N-PEG-NH2 and folate by active ester method, whereafter, active- targeting nanoparticles were prepared through solution volatilization and self-assembly. 1H NMR was employed to characterize the structure of copolymer, and luminescence spectrometer was characterized the diluting stabili ty of nanoparticles, then model cells breast cancer cells （MCF-7） and fibroblasts cells （CCL-110） were to evalu- ate the targeting- mediated endocytosis pathway. The results showed that the nanoparticles have good diluting stability and targeting selectivity on the basis of successful synthetic materials, and these are potential to be- come the receptor mediated targeted drug release system carrier material.
同期刊论文项目
同项目期刊论文
Feasibility study for thermal protection by microencapsulated phase change micro/nanoparticles durin
Comparative Study of Normal and Rheumatoid Arthritis Fibroblast-Like Synoviocytes Proliferation unde
Migration of Human Mesenchymal Stem Cells Under Low Shear Stress Mediated by Mitogen-Activated Prote
Effects of hydroxyapatite/collagen composite on osteogenic differentiation of rat bone marrow derive
Mechano-growth factor induces migration of rat mesenchymal stem cells by altering its mechanical pro
Differential response to CoCl2-stimulated hypoxia on HIF-1 alpha, VEGF, and MMP-2 expression in liga
Conditioned medium from hepatocellular carcinoma cells promotes mesenchymal stem cells migration thr
Functional and transcriptomic analysis of the regulation of osteoblasts by mechano-growth factor E p
Electrospun natural- artificial synthetic polymer blended nanofibers and their influence on cellular
Mesenchymal stem cells require integrin beta 1 for directed migration induced by osteopontin in vitr
Low-Level Shear Stress Induces Human Mesenchymal Stem Cell Migration Through the SDF-1/CXCR4 Axis Vi
Dodecanol-poly(D,L-lactic acid)-b-poly (ethylene glycol)-folate (Dol-PLA-PEG-FA) nanoparticles: Eval
RhoA/ROCK, cytoskeletal dynamics, and focal adhesion kinase are required for mechanical stretch-indu
In vitro cytocompatibility evaluation of MGF-Ct24E chemically grafted and physically blended with ma
Human iPSC-Derived Neural Crest Stem Cells Promote Tendon Repair in a Rat Patellar Tendon Window Def
Combined Effects of Mechanical Strain and Hydroxyapatite/Collagen Composite on Osteogenic Differenti
Ectopic osteogenesis of a microsphere-scaffold delivery system with encapsulated synthetic peptide d
Mechano growth factor E peptide regulates migration and differentiation of bone marrow mesenchymal s
Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbit
Research Advancement on Injured Peripheral Nerve Regeneration by Stem Cells Combined With Electrical
Mechanical stretch-induced f-actin reorganization and tenogenic differentiation of human mesenchymal
High-level Shear Stress Stimulates Endothelial Differentiation and VEGF Secretion by Human Mesenchym
A synthetic mechano-growth factor E peptide promotes rat tenocyte migration by lessening cell stiffn
Effect of Internal Structure of Collagen/hydroxyapatite Scaffold on the Osteogenic Differentiation o
A novel positively thermo-sensitive hydrogel based on ethylenediaminetetraacetic dianhydride and pip
Microencapsulation of mechano growth factor E peptide for sustained delivery and bioactivity mainten
Effect of Focal Adhesion Kinase on the Regulation of Realignment and Tenogenic Differentiation of Hu
Design, synthesis and characterization of novel biodegradable shape memory polymers based on poly(D,
Degradation studies on segmented polyurethanes prepared with poly (D, L-lactic acid) diol, hexamethy
Role of FAK-ERK1/2 Signaling Pathway in Proliferation of Rat Bone-marrow Mesenchymal Stem Cells Stim
Matrix Mechanics and Fluid Shear Stress Control Stem Cells Fate in Three Dimensional Microenvironmen
Three-Dimensional Morphometric Comparison of Normal and Apoptotic Endothelial Cells Based on Laser S
Conditioned medium from mesenchymal stem cells enhances the migration of hepatoma cells through CXCR
期刊信息
位置：