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Surface Modification of NiTi Alloy via Cathodic Plasma Electrolytic Deposition and its Effect on Ni Ion Release and Osteoblast Behaviors＊

ISSN号：1009-0630
期刊名称：《等离子体科学与技术：英文版》
时间：0
分类：Q25[生物学—细胞生物学] O614.33[理学—无机化学;理学—化学]
作者机构：[1]Key Laboratory of Biorheological Science and Technology of Ministry of Education,College of Bioengineering, Chongqing University, Chongqing 400044, China
相关基金：supported by China Ministry of Science and Technology （973 project No. 2009CB930000）, National Natural Science Foundation of China （Nos. 11032012 and 51173216）, Fok Ying Tung Education Foundation （121035）, Natural Science Foundation of Chongqing Municipal Government （CSTC2011jjjql0004 and CSTC2012gg-yyjs10023）, Fundamental Research Funds for the Central Universities （Nos. CDJXS10232211, CDJZRl1230005）, and the sharing fund of Chongqing University＇s large-scale equipment （Nos. 2011063046, 2011063047）

作者：燕颖[1], 蔡开勇[1], 杨维虎[1], 刘鹏[1]

关键词：等离子体质谱法, NITI合金, 成骨细胞分化, 表面改性, 电解沉积, 镍离子, 细胞行为, 阴极, surface modification, plasma applications, biomaterials, cathodic plasma elec-trolytic deposition

中文摘要：

To reduce Ni ion release and improve biocompatibility of NiTi alloy, the cathodic plasma electrolytic deposition (CPED) technique was used to fabricate ceramic coating onto a NiTi alloy surface. The formation of a coating with a rough and micro-textured surface was confirmed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, respectively. An inductively coupled plasma mass spectrometry test showed that the formed coating significantly reduced the release of Ni ions from the NiTi alloy in simulated body fluid. The influence of CPED treated NiTi substrates on the biological behaviors of osteoblasts, including cell adhesion, cell viability, and osteogenic differentiation function (alkaline phosphatase), was investigated in vitro. Immunofluorescence staining of nuclei revealed that the CPED treated NiTi alloy was favorable for cell growth. Osteoblasts on CPED modified NiTi alloy showed greater cell viability than those for the native NiTi substrate after 4 and 7 days cultures. More importantly, osteoblasts cultured onto a modified NiTi sample displayed significantly higher differentiation levels of alkaline phosphatase. The results suggested that surface functionalization of NiTi alloy with ceramic coating via the CPED technique was beneficial for cell proliferation and differentiation. The approach presented here is useful for NiTi implants to enhance bone osseointegration and reduce Ni ion release in vitro.

英文摘要：

To reduce Ni ion release and improve biocompatibility of NiTi alloy, the cathodic plasma electrolytic deposition （CPED） technique was used to fabricate ceramic coating onto a NiTi alloy surface. The formation of a coating with a rough and micro-textured surface was confirmed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, re- spectively. An inductively coupled plasma mass spectrometry test showed that the formed coating significantly reduced the release of Ni ions from the NiTi alloy in simulated body fluid. The in- fluence of CPED treated NiTi substrates on the biological behaviors of osteoblasts, including cell adhesion, cell viability, and osteogenic differentiation function （alkaline phosphatase）, was inves- tigated in vitro. Immunofluorescence staining of nuclei revealed that the CPED treated NiTi alloy was favorable for cell growth. Osteoblasts on CPED modified NiTi alloy showed greater cell viability than those for the native NiTi substrate after 4 and 7 days cultures. More importantly, osteoblasts cultured onto a modified NiTi sample displayed significantly higher differentiation lev-els of alkaline phosphatase. The results suggested that surface functionalization of NiTi alloy with ceramic coating via the CPED technique was beneficial for cell proliferation and differentiation. The approach presented here is useful for NiTi implants to enhance bone osseointegration and reduce Ni ion release in vitro.