中文摘要：

背景：目前慢病毒介导胰岛素生长因子1基因作用于人骨髓间充质干细胞的研究还处在初级阶段。目的：构建携带胰岛素生长因子1基因的慢病毒载体，观察其在人骨髓间充质干细胞中的表达以及其对成骨分化的影响。方法：应用重组慢病毒技术将胰岛素生长因子1基因和绿色荧光蛋白转染至体外培养第3代人骨髓间充质干细胞（实验组），以空白的慢病毒转染的人骨髓间充质干细胞和未做处理的人骨髓间充质干细胞分别作为对照组和空白组进行对比。结果与结论：慢病毒转染3d后，对照组和实验组的转染效率分别可达95％和90％以上。Westernblot检测结果和激光共聚焦显微镜下观察显示，胰岛素生长因子1在人骨髓间充质干细胞中成功表达。说明胰岛素生长因子1基因转染对人骨髓间充质干细胞成骨能力具有促进作用。

英文摘要：

BACKGROUND： Research on bidirectional regulation effects of human insulin-like growth factor-1 （hlGF-1） gene induction on stem cells is still in primary phase currently. OBJECTIVE： Lentivirus vector carrying hlGF-1 gene were used to transfect human bone marrow mesenchymal stem cells, and then to observe the expression of target gene and differentiation of those cells. METHODS： The recombinant lentiviral vectors carrying hlGF-1 gene and green fluorescent protein were constructed with recombinant lentiviral technology, and then the recombinant lentiviral vectors were used to transfect the passage 3 human bone marrow mesenchymal stem cells in vitro cultured （transfection group）. The bone marrow mesenchymal stem cells transfected with lentivirals were as control （control group）. RESULTS AND CONCLUSION： At 3 days after trasfection, the green fluorescence bone marrow mesenchymal stem cells could be observed under fluorescence microscope, and the transfection efficiency of control group was over 95%,while the transfection group was over 90%. Confocal laser scanning microscope and Western blot results showed that hlGF-1 gene have the positive effect of promoting expression in cellular and protein level of human bone marrow mesenchymal stem cells.