中文摘要：

试验建立了黑曲霉（TR-H）在基础培养基中对氯嘧磺隆除草剂降解的提取和检测方法。结果表明：采用二氯甲烷作为提取溶剂，氯嘧磺隆在基础培养基中的添加回收率均大于96．O％，标准偏差均小于4．0％。采用甲醇-水（pH2．5，用85．0％的磷酸调节）作为液相色谱的流动相体系，可将氯嘧磺隆与其它物质较好的分离。氯嘧磺隆的浓度在0．1～200．0mg／L范围内具有良好的线性关系，相关系数为r=0．9998（n=3）。氯嘧磺隆在基础培养基中添加的最低浓度应在0．05mg／L（S／N=3）以上方能被检出；此外，氯嘧磺隆在菌体上的吸附均低于其检出限。

英文摘要：

Methods of extracting and determining chlorimuron-ethyl herbicide degraded by aspergillus niger（TR-H） in culture medium was established. The results showed that ： （1）using dichloromethane as extracting solvent, the fortified recovery of chlorimuron-ethyl in basal medium was more than 96.0% and the standard deviation was less than 4. 0%. （2）using methanol - water （pH value adjusted to 2.5 with 85.0% phosphoric acid） as mobile phase, chlorimuron-ethyl and other substances were well separated. （3） a good linear range from 0.1 - 200.0mg/L concentration of chlorimuron- ethyl with correlation coefficient of 0.9998 （ n = 3 ） was obtained. （4） the lowest deteetion limit of chlorimuron-ethyl in basic culture medium was 0.05mg/L（ S/N = 3） and the adsorption of chlorimuron-ethyl on aspergiliuse niger was low- er than the lowest detection limit.