中文摘要：

采用紫外（UV）诱变方法对黑曲霉进行诱变,用高效液相色谱法测定菌株对氯嘧磺隆的降解率,系统研究了黑曲霉经紫外诱变后对氯嘧磺隆降解能力的影响。结果表明：经紫外（UV）诱变黑曲霉总变异率随UV强度和辐照时间的不同而不同,受时间影响较大,对黑曲霉最佳的紫外（UV）诱变条件为UV强度30 W、辐照时间150 s,此条件下黑曲霉的正变率为35.16%;紫外（UV）诱变可以显著提高黑曲霉降解氯嘧磺隆的速度,在UV强度30 W、时间150 s时黑曲霉对氯嘧磺隆的降解速度最大,氯嘧磺隆半衰期为7.76 h比原始菌株（24.86 h）缩短17.1 h。最终筛选出降解能力提高最显著的菌株H1,其降解能力比原始菌株提高73.16%,且连续培养10代后降解率均在89%左右,降解性能及生长速度稳定,可以稳定遗传。

英文摘要：

In this paper,the effect of ultraviolet mutagenesis on chlorimuron-ethyl degradation ability of Aspergillus Niger was systematic studied. Ultraviolet（ UV） method is used to Aspergillus Niger mutagenesis,and a high performance liquid chromatography method was established for determination of the degradation rates of stain to chlorimuron-ethyl. The result showed that,induced mutation by ultraviolet（ UV） the total aberration rates of Aspergillus Niger was varied with the UV intensity and irradiation time,greatly influenced by time,the best UV condition was 30 W and 150 s,positive mutation rate was 35. 16%.The degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger was studied after UV mutagenesis. UV mutagenesis could significantly improve degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger,When the UV condition was30 W and 150 s the degradation rate of Aspergillus Niger on chlorimuron-ethyl was the greatest,half-life of chlorimuron-ethyl shortened 17. 1 h than before. Finally selected the most significant effect strains H1,its degradation ability increased by73. 16% than the original strains,and continued training after 10 generations degradation of performance and growth rate were stable and could be stable genetic.