中文摘要：

目的建立小鼠非清髓性单倍体相合骨髓移植模型,探讨移植前输注供体细胞的造血干细胞植入效果。方法以CB6F1雌性小鼠为受鼠,C57BL/6雄性小鼠为供鼠,移植前3d经尾静脉输注供鼠脾细胞或骨髓细胞3×107,输注细胞后24h和48h分别通过腹腔注射阿糖胞苷0.015g/d,移植前1d予450cGy全身照射（TBI）,移植当天输注供鼠骨髓有核细胞5×107/只。监测受鼠白细胞恢复、Y染色体性别决定基因（SRY）以及外周血供鼠CD3＋细胞嵌合状态。结果单纯给予TBI小鼠均存活,且移植后30d白细胞恢复正常,TBI＋骨髓细胞移植（TBI＋BMT）组移植后14、30、60d时外周血SRY基因PCR检测结果均阳性,60d供体CD3^＋细胞嵌合率达54.4%。移植前输注供体脾细胞组嵌合率最高,移植后60d达93.5%±4.8%,优于移植前输注供体骨髓细胞组（P〈0.05）。结论 450cGy TBI的非清髓性预处理可以成功建立非清髓性单倍体相合骨髓移植模型,移植前输注供体脾细胞可促进造血干细胞植入。

英文摘要：

Objective To establish the mouse model of non-myeloablative haploidentical bone marrow transplantation（BMT） and study the outcome of donor hemopoietic stem cells transfusion before haploidentical BMT.Methods CB6F1 female mice were used as recipients and C57BL/6 male mice were used as donors.Three days before BMT,3×107 donor spleen cells or bone marrow cells were transfused through the tail vein.Then,Ara-C（0.015g/d） was injected into abdominal cavity 24h and 48h after transfusion of spleen cells or bone marrow cells.One day before BMT,the recipients received total body irradiation（TBI） at the dose of 450cGy.On the day of BMT,5×107 nucleared bone marrow cells from male donor mice were transfused.Restore of white blood cells and sex determining region Y gene（SRY） in recipients and chimerism in CD3＋ cells of donors after BMT were monitored.Results The mice after simple TBI survived and their white blood cells became normal 30 days after BMT.PCR showed the SRY in peripheral blood 14,30 and 60 days in mice after TBI and BMT.The chimerism of CD3＋ cells in donors on day 60 was 54.4% before BMT and 93.5%±4.8% after transplantation of spleen cells（P〈0.05）.Conclusion Non-myeloablative pretreatment with 450cGy TBI can establish the mouse model of non-myeloablative haploidentical BMT and transfusion of donor spleen cells before BMT can promote transplantation of hemopoietic stem cells.