中文摘要：

目的：检测白血病细胞系NK配体表达情况以及对NK-92细胞的敏感性,探讨NK配体表达与NK杀伤敏感性的关系。方法：通过半定量RT-PCR的方法,以β-actin为内参基因,分别检测U937、CEM、KG-1a、HL-60、NB4、Reh和LCL等7种细胞表面MICA、MICB、ULBP1、PVR、Nectin-2、LFA-3、LLT-1、HLA-E、HLA-F和HLA-G等10种NK配体的表达情况。通过流式细胞术,用CFSE和PI双染法,检测NK-92细胞对以上7种白血病细胞系的杀伤效应。结果：根据NK-92细胞对靶细胞的杀伤效应强弱,将以上7种白血病细胞系分成NK敏感组（U937,CEM,KG-1a）、中度敏感组（HL-60、NB4）和不敏感组（Reh,LcL）三组。NK配体PVR和HLA-F在组间的差异具有统计学意义（P值分别为0.017和0.016）,不敏感组的PVR转录水平最低而HLA-F水平最高,其他配体间则无显著性差别。结论：本研究初步表明PVR和HLA-F是人为干预肿瘤细胞耐受NK细胞的两个靶点分子。

英文摘要：

Objective：To detect the expression of NK ligands in leukemia cell lines and their sensitivity to NK-92 respectively.And analyze the association between NK ligands’ expression and NK cytotoxicity.Methods：Using β-actin as control gene,10 NK ligands（MICA,MICB,ULBP1,PVR,Nectin-2,LFA-3,LLT-1,HLA-E,HLA-F,HLA-G） in 7 leukemia cell lines（U937,CEM,KG-1a,HL-60,NB4,Reh,LCL） respectively were detected by semi-quantitative RT-PCR method.With CFSE and PI double staining,the NK-92 cytotoxicity against the cell lines were analyzed by flow cytometry.Results：Leukemia cell lines were classified into three groups（sensitive,moderate and insensitive） according to their sensitivity to NK-92 cytotoxicity.U937,CEM and KG-1a were sensitive group,HL-60 and NB4 were moderate group,Reh and LcL were insensitive group.The transcriptional levels of PVR and HLA-F were statistically different among groups（P value was 0.017 and 0.016 respectively）.PVR of insensitive group was the lowest while HLA-F of which was the highest.Conclusion：Our study preliminary show that PVR and HLA-F are the potential targets through which we could highly increase the NK effectors in adoptive immunotherapy.