中文摘要：

建立高效液相色谱（HPLC）测定麦糟水解液中的单糖及脂肪酸的分析方法，检测条件：色谱柱为Aminex HPX-87H柱，柱温为50℃，流动相为5mmol·L^-1的硫酸，流速为0．4mL·min^-1，检测器为示差折光检测器．在此条件下，水解液中的单糖及脂肪酸的峰面积与浓度线性关系良好，相关系数R。在0．99991～0．99998之间，回收率为97．3％～101．5％．通过对样品的分析表明，麦糟水解液中不含有葡萄糖、甲酸及乙酰丙酸，木糖和阿拉伯糖的质量浓度比较高，乙酸的质量浓度比较低，单糖及脂肪酸的混合液得到很好的分离，分离过程在23min内完成．

英文摘要：

A method for analysis of monosaccharides and fatty acid in the liquors of acid hydrolysis on Brewery＇s spent grain by high performance liquid chromatography （HPLC） is described. The operating conditions were Aminex HPX-87H column at 50℃ and differential refractive index detector, the mobile phase was 5 mmol· L^-1 H2SO4 and the flow-rate was 0.4 mL · min^-1. The monosaccharides and fatty acid in the liquors was measured in the anterior conditions. The correlation coefficient was 0. 999 91～0. 999 98 and the corresponding average recoveries were 97.3%～101.5%. Glucose, formic acid and levulinic acid were not detected in the liquors of acid hydrolysis and the concentration of xylose and arabinose was high, while the acetic acid was low. The mixture of monosaccharides and fatty acid was efficiently separated and the whole process of separation was finished within 23 minutes.