中文摘要：

目的研究融水小型猪的线粒体DNA结构和系统进化关系。方法选取了全基因组鸟枪法的测序策略对融水小型猪的外周血液样本进行测序,使用Illumina基因组分析测序技术构建paired-end文库进行末端测序,最后进行拼接及分析。结果融水小型猪的完整线粒体序列呈环形,总长为16888 bp,由13个编码蛋白基因、22个转运RNA和两个核糖体RNA构成。线粒体DNA的GC含量约为44%。系统进化分析、遗传多样性分析和中性检验证明了,在融水小型猪、兰屿猪、海南野猪和滇南7号这一类群中,发生了种群扩张。结论融水小型猪是中国传统猪种中比兰屿猪更为古老的小型猪。

英文摘要：

Objective Assembly whole mitochondrial genome sequence of Rongshui miniature pig （ RMP ） breed and analysis the structure of mitochondrion based on the next-generation sequecing method. Comparison of phylogenetic relationship and genetic diversity among different pig breeds. Methods We collected peripheral venous blood sample from RMP and constructed two paired-end sequencing libraries. A whole-genome shotgun sequencing strategy and Illumina Genome Analyser sequencing technology were used in our study. Results The mitochondria] genome of RMP consists of 13 protein coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and the length of pig is 16888 bp. The GC content of this pig mitochondrial genome is about 44 %. Based on phlogenetic analysis, population genetic analysis, our findings confirmed that the ancestral cluster in East Asia mainly occurred among Diannan 7# pig, Hainan wild boar, Lanyu and RMP. Conclusion RMP, a typical miniature pig breed in China, is an earlier ancestor than Lanyu pig breed.