中文摘要：

目的：探讨补肾健脾中药健骨颗粒对体外破骨细胞整合素（Itg）αV、β3mRNA表达的影响。方法：采用破骨前体细胞系诱导培养法,通过M-CSF和RANKL诱导剂诱导RAW264.7细胞,分化为成熟破骨细胞。分别用健骨颗粒含药血清和生理盐水血清干预,运用实时荧光定量PCR法检测ItgαV、β3mRNA表达,并取骨片行甲苯胺蓝染色,骨陷窝和骨吸收面积计算分析。结果：经M-CSF和RANKL诱导后,RAW264.7细胞的形态特征、TRAP染色均符合成熟破骨细胞特异性表现;含药血清组破骨细胞ItgαV、β3mRNA表达水平明显低于生理盐水血清组（P〈0.05）,同时骨磨片的骨吸收陷窝数和面积亦呈同样变化趋势。结论：RAW264.7细胞经诱导分化后可以获取成熟破骨细胞;健骨颗粒能有效抑制破骨细胞Itgαv、β3 mRNA表达,影响破骨细胞功能活性。

英文摘要：

Objective： To investigate the effects of Jiangu grunula（JGG） of traditional Chinese drug which tonifying the kidney and benefiting the spleen on the expression of integrin αv, β3 in osteoclast in vitro. Methods： The preosteoclasts of RAW264.7 cell were induced to mature osteoclast by RANKL and M-CSF. Then cultured the mature osteoclast and the JGG or normal saline（NS） contained serum were respectively added. The expression of Itg αv, β3 was measured by real time RT-PCR. Then the number and area of bone resorption pits on bone slices stained by methods of Toluidine Blue were counted. Results： The RAW264.7 cell, which induced by RANKL and M-CSF, consistent with morphological characteristies of mature osteoclast, or stained by TRAP method. The Expression of Itg αv, β3 in osteoclast of JGG-group was significantly lower than NS-group（P〈0.05）, and same as the number and area of bone resorption pits on bone slices. Results： RAW264.7 cell can be induced to mature osteoclast. JGG can effectively inhibit the expression of Itg αv, β3 in osteoclast, and then influence the function of osteoclast.