中文摘要：

目的通过检测早期类风湿关节炎（RA）患者血清中的白细胞介素（IL）-33水平，分析其与早期RA之间的相关性。方法收集病程〈1年的早期RA患者100例，骨关节炎（OA）患者40例以及健康对照者70名。采用双抗体夹心酶联免疫吸附试验（ELISA）测定血清中的IL-33水平，并分析血清IL-33水平与RA各临床和实验室指标的相关性。计量资料的比较采用Kruskal—Wallis检验和（或）Mann—Whitney U检验，计数资料比较采用矿检验，相关性分析采用Spearman相关分析。结果RA患者血清IL-33水平为（282±871）pg／ml，显著高于健康对照组[（7±38）pg／ml，P〈0．01]和OA患者[（8±35）pg／ml，P〈0．01]。血清IL-33水平与类风湿因子（RV）、隐性类风湿因子IgG（HRF—IgG）、抗环瓜氨酸肽（CCP）抗体、抗突变型瓜氨酸化波形蛋白（MCV）抗体呈正相关（r分别为：0．312，0．277，0．213，0．302，P〈0．01或P〈0．05）。IL-33阳性组患者的RF阳性率、HRF—IgG阳性率、抗CCP抗体阳性率、抗MCV抗体阳性率（86％、31％、86％、94％）较IL-33阴性组患者（54％、11％、42％、72％）显著升高（P均〈0．05）。结论IL-33在RA患者血清中显著升高，并与多种自身抗体（包括RF、抗CCP抗体、抗MCV抗体和HRF—IgG）显著相关。可能是RA预后不良的因素之一。

英文摘要：

Objective To analyze the correlation between serum interleukin （IL）-33 and early rheumatoid arthritis （RA） by testing the serum IL-33 of patients with RA. Methods One hundred patients with early RA＇whose disease duration was less than 1 year were selected, 40 patients with osteoarthritis （OA） and 70 healthy controls were selected. The serum IL-33 levels of the three groups were detected by enzyme-linked immunosorbent assay （ELISA）. The correlation between serum IL-33 levels and clinical features of patients with HA were analyzed. Kruskal-Wallis test, Mann-Whitney U test, X2 test and Spearman correlation analysis were used for statistical analysis. Results The level of serum IL-33 in RA patients [（282±871 ） pg/ml] was significantly higher than that of the healthy controls [ （7±38） pg/ml, P〈0.01 ] and OA patients [ （8±35） pg/ml, P〈0.01]. The levels of serum IL-33 in RA patients were related with rheumatoid factor （RF）, occult rheumatoid factor immunoglobnlin G （HRF-IgG）, anti-cyclic citrullinated peptide antibodies （anti-CCP）, anti-mutated citrullinated vimentin antibodies （anti-MCV）. The positive rate of RF, HRF-IgG, anti-CCP and anti-MCV in IL-33 positive group （86%, 31%, 86%, 94%） was significantly higher than that of the IL-33 negative group （54%, 11%, 42%, 72%） （P〈0.05）. Conclusion The level of serum IL-33 is significantly elevated in RA and it is significantly associated with some of the auto-antibodies （including RF, anti-CCP antibodies, anti-MCV antibodies and HRF-IgG）. Thus, IL-33 may be a factor for unfavorable prognosis to RA patients.