中文摘要：

目的 应用MR多技术扫描评价转染血管内皮生长因子的自体骨髓基质细胞移植于慢性缺血动物模型猪后对心功能的保护和促血管新生作用。方法 将慢性缺血心脏病模型猪随机分2组：注射转染血管内皮生长因子基因的自体骨髓基质细胞组（组Ⅰ，n=9）和注射空腺病毒载体的对照组（组Ⅱ，n=7）。治疗后4周应用MR多技术扫描检测各实验组射血分数、缺血坏死节段，取心脏标本测定梗塞区的百分比、荧光显微镜下标记细胞、血管数量。结果 与组Ⅱ相比组Ⅰ的射血分数明显提高，缺血坏死面积明显减少（P〈0．05），且荧光显微镜下可见大量CM-DiⅠ标记的移植存活细胞。组Ⅰ缺血区血管计数高于组Ⅱ并有显著性差异。结论 携带血管内皮生长因子的自体骨髓基质细胞移植治疗缺血性心脏病具有明显的优越性，移植细胞成活率高，心功能和局部血运改善明显。

英文摘要：

Objective To investigate the evaluation of multimodality MR imaging on the transplantation of autologous marrow stromal cells transfected ex vivo by vascular endothelial growth factor （ VEGF ） gene in a porcine chronic ischemic heart disease model. Methods Pigs of chronic ischemic heart disease model were randomly divided into two groups ： treatment with marrow stromal cells transfected ex vivo by VEGF （ Group Ⅰ, n = 9 ） and treatment with adenovirus served as control （ Group Ⅱ , n = 7）. Four weeks after therapy, ejection fraction, ischemic segment and infracted segment were detected by multimodality MR imaging. The percentage of infarction area, CM -DiI labeled cells on fluorescence mlcroscopy, vessel density were detected on specimen. Results In Group Ⅰ ,in comparison with Group Ⅱ , the ejection fraction was significantly improved, the ischemie and infarction segments decreased （ P 〈 0. 05）. There was successful engraftment of marrow stromal cells in Group I as shown by the CM - DiⅠ labeled cells seen at the site of implantation on fluorescence microscopy. The vessel number had significantly difference between Group Ⅰ and Group Ⅱ. Conclusion Transplantation of autologous marrow stromal cells transfected ex vivo by VEGF gene offers obvious advantages of the diffused survival of implanted cells and the blood supply as well as heart function has significantly improvement.