中文摘要：

目的应用MR评价转染血管生长素（ANG）的自体骨髓基质细胞（MSCs）移植于慢性缺血性心脏病动物模型猪后对心功能的保护和促血管新生作用。材料与方法将30只猪随机分3组,共有8只猪在建立动物模型的过程中死亡。剩余22只随机分为：注射转染ANG的自体MSCs组（组Ⅰ,n=8）、注射单纯转染ANG组（组Ⅱ,n=7）和注射空腺病毒载体的对照组（组Ⅲ,n=7）。处理后4周应用MR扫描检测各实验组射血分数,缺血、坏死及运动异常节段,荧光显微镜下标记细胞、血管数量。结果组Ⅰ、组Ⅱ治疗后射血分数明显提高,缺血节段明显减少（P〈0.05）,3组处理前后坏死、运动异常节段均无明显差异（P〉0.05）。荧光显微镜下组Ⅰ缺血区内可见大量CM-DiI标记的移植存活细胞。组Ⅰ、组Ⅱ缺血区血管计数高于组Ⅲ并有统计学差异（P〈0.05）。结论携带ANG的自体MSCs移植治疗缺血性心脏病具有明显的优越性,移植细胞成活率高,心功能和局部血运改善明显。

英文摘要：

Objective To evaluate the role of MR imaging on the transplantation of autologous marrow stromal cells transferred ex vivo by angiogenin in a porcine chronic ischemic heart disease model. Materials and Methods 30 Pigs were randomized to 3 groups,8 pigs died in process of animal model establishment, remaining 22 pigs were randomized to： treatment with marrow stromal cells transferred ex vivo by angiogenin （ Group Ⅰ, n = 8）, treatment with angiogenin gene transfer（ Group Ⅱ, n = 7 ） and treatment with adenovirus served as control （ Group Ⅲ, n = 7 ）. Four weeks after management, ejection fraction, ischemic segment and infracted segment were detected by magnetic resonance imaging. CM DiI labeled cells on fluorescence microscopy, vessel density were detected on specimen. Results Group Ⅰ and group Ⅱ showed significantly improvement in the ejection fraction and ischemic segments significantly decreased （ P 〈 0.05 ）. Necrosis and motion abnormality segments had no significantly difference between pre and post management in three group （P 〉 0.05 ）. There was successful engraftment of marrow stromal cells in group Ⅰ as shown by the CM DiI labeled ceils seen at the site of implantation on fluorescence microscopy. Vessel number detected in Group Ⅰ and Group Ⅱ were significantly higher than that in Group Ⅲ （P 〈 0. 05）. Conclusion Transplantation of autologous marrow stromal cells transferred ex vivo by angiogenin gene offers obvious advantages of the diffused survival of implanted ceils and the blood supply, and the heart function has significantly be improved.