中文摘要：

目的：建立实时定量PCR检测Elf-1基因表达水平的方法，了解急性髓细胞性白血病（AML）患者外周血Elf-1基因表达水平。方法：采用SYBRGreenI荧光定量PCR和相对定量分析法检测33例AML患者：M2型17例、M3型6例、M5型10例和20例健康人外周血的单核细胞的Elf-1表达情况，以p2微球蛋白基因（β2M）作为内参，采用公式2-△Ct×100％计算Elf-1基因表达水平。结果：AML组Elf-1表达水平（13．518±19．197）％明显高于健康对照组（2．044±1．321）％（P〈0．01），不同AML亚型之间Elf-1的表达水平没有显著性差异（P=0．52），但各亚型AML的Elf-1的表达水平均与健康对照组比较均有显著性差异（P〈0．01）。结论：建立SYBRGreenI荧光实时定量PCR分析外周血Elf-1表达水平的方法，检测急性髓细胞性自血病Elf-1基因高表达情况。

英文摘要：

Aim：To establish a real-time PCR technique for detection and quantify of Elf-1 gene expression and to investigate the expression level of Elf-1 gene in patients with acute myeloid leukemia （AML）. Methods： Real-time PCR with SYBR Green I technique was used for detecting Elf-1 expression level in peripheral blood mononuclear cells of 33 patients with AML （including 17 cases with M2, 6 cases with M3, 10 cases with M5 and 20 healthy individuals）. β2-microglobulin gene （β2M） was used as an endogenous reference. Relative changes in Elf-1 expression level were used by the 2-△Ct×100% method. Results： The expression level of Elf-1 in 33 cases with AML（ 13. 518 ± 19. 197% ） was significant higher than those in the healthy control （2. 044 ± 1.321% ） （P 〈0. 01 ）. The expression level of Elf-1 had not significant difference among the three subtypes （P = 0. 52）. However, by comparing with the healthy control, the expression level of Elf-1 had significant difference in all of three subtypes （ P 〈 0. 01 ）. Conclusion：The SYBR Green I real-time technique for quantitative detection of Elf-1 expression levels was established. And the overexpression of Elf-1 gene in AML was detected.