采用PHA结合秋水仙索活体注射。取条纹斑竹鲨(Chiloscyllium plagiosum)脾脏经低渗处理,空气干燥制片法制作染色体标本,对染色体进行Giemsa染色,完成条纹斑竹鲨染色体核型分析.研究结果表明,条纹斑竹鲨的染色体组由20对中部着丝粒染色体、12对亚中部着丝粒染色体、5对亚端部着丝粒染色体和14对端部着丝粒染色体构成,染色体核型公式为:2n=102=28t+10st+24sm+40m,染色体臂数NF=166,其中1对亚中部着丝粒染色体带有明显的次缢痕.与其他软骨鱼类相似,条纹斑竹鲨同样具有数目众多的染色体二倍体数,但以中部着丝粒和亚中部着丝粒染色体为主.
The chromosome specimens of the lip shark Chiloscyllium plagiosum were obtained from the spleen cell by vivo injection of P HA and cultivation of colchicines,hypatoic-air drying technique, and then,by studying their Giemsa stain,the analysis of the karyotype was completed. According to the chromosome centromeric index values found,the karyotype of C. plagiosum is composed of 20 metacentric, 12 metacentric-suhmetacentric,5 telocentric- suhtelocentric and 14 telocentric pairs, and the karyotype formula is: 2n= 102= 28t+ 10st+24sm+ 40m. NF= 166, and there was one pair of metacentric-suhmetaeentric chromosomes with secondary constriction. Compared to the other cartilaginous fishes, C. plagiosum had high diploid chromosome number, most of which were metacentric and metacentric-submetacentric chromosomes.