Mechanism of the Rpn13-induced activation of Uch37
- ISSN号：1674-800X
- 期刊名称：Protein & Cell
- 时间：2014.8
- 页码：616-630
- 分类：Q556.2[生物学—生物化学] TP317.1[自动化与计算机技术—计算机软件与理论;自动化与计算机技术—计算机科学与技术]
- 作者机构：[1]National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China, [2]iHuman Institute, ShanghaiTech University, Shanghai 201210, China, [3]Shandong Provincial Key Laboratory ofEnergy-Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences, Qingdao 266101, China, [4]Physics Division,'Los Alamos National Laboratory, Los Alamos, NM 87545, USA, [5]X-ray Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA, [6]Institute of Crystallography~ Russian Academy of Sciences, 59 Leninsky Pr., 117333 Moscow, Russian Federation, [7]Center for Multi-disciplinary Research, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049,China, [8]Department of Nerosurgery, The Military General Hospital of Beijing PLA, Beijing 100700, China
- 相关基金：This work was supported by the National Basic Research Program （973 Program） （Nos. 2014CB910400 and 2013CB911103）, the Ministry of Health of China （Grant No. 2013ZX10004-602）, National Key Technology Research and Development Program of the Ministry of Science and Technology of China （Grant No. 2014BAI07B02） and the National Natural Science Foundation of China （Grant Nos. 31330019, 31200559）. We would like to thank Dr. Li-Qin Li from the Institute of High Energy Physics, CAS and Professor Robert E. Cohen for the gen- erous gift of the hUch37 （C88A） plasmid and Xiaoxia Yu and Yu- anyuan Chen at the Protein Science Core Facility of IBP for their technical help with the AUC and SPR experiments. The authors would also like to thank the staff at beamline BL13.3.1 at ALS for their technical support with the SAXS data collection. BL13.3.1 is supported in part by the DOE program Inte- grated Diffraction Analysis Technologies （IDAT） and the DOE pro- gram Molecular Assemblies Genes and Genomics Integrated Efficiently （MAGGIE） under Contract Number DE-AC02-05CH11231 with the DOE. The ALS is supported by the Director, Office of Sci- ence, Office of Basic Energy Sciences of the DOE under Contract No. DE-AC02-05CH11231. Use of the Advanced Photon Source, an Office of the Science User Facility operated for the U.S. Department of Energy （DOE） Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02- 06CHl1357. BioCAT was supported by grants from the National Center for Research Resources （2P41RR008630-17） and the National Institute of General Medical Sciences （9 P41 GM103622- 17） from the National Institutes of Health. The authors would like to thank the staff at 121D and 181D for the setup support.
- 相关项目：天然免疫系统感受因子STING与c-di-GMP复合物结构解析及TBK1激活机制研究
关键词：
激活机制, 催化结构域, 诱导, 蛋白酶体, 自动抑制, 活性位点, SAXS, X射线散射, Uch37-Rpn13 complex, de-ubiquitination,SAXS analysis, oligomerization, iso-peptidase
中文摘要：
Correspondence： zjxuruxiang@163.com （R. Xu）,
zjliu@ibp.ac.cn （Z.-J. Liu）
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