中文摘要：

以胆固醇琥珀酸单酯对壳聚糖进行仿生功能化改性,并利用红外光谱和核磁共振波谱对改性后产物进行了表征.通过接触角测试、仿生矿化及细胞培养等方法研究了改性后产物的相关性能.结果表明,改性后的壳聚糖衍生物接触角有所增大.在仿生矿化过程中,随着时间的延长,壳聚糖衍生物具有对沉积的钙磷盐的稳固作用.在细胞培养过程中,改性后的壳聚糖膜上细胞增殖明显.噻唑蓝（MTT）比色法、扫描电子显微镜（SEM）与激光共聚焦显微镜（CLSM）表征结果表明,改性后的壳聚糖膜具有促进3T3细胞黏附、伸展与增殖的效果.

英文摘要：

Cholesterol-modified chitosan conjugate with succinyl linkages（CHCS） was synthesized and cha-racterized by Fourier transform infrared（FTIR） and proton nuclear magnetic resonance（1H NMR） spectra.The extent of grafting cholesterol was characterized by elemental analyzer.The properties of CHCS and chitosar（CS） were studied by water contact angle test,biomineralization and cell culture.The results show that the contact angle of CHCS is larger than that of CS.In the process of biomineraliation,CHCS can stable the deposited calcium phosphorus salt.The relationships between CHCS/CS and 3T3 cells were studied by MTT method and cytoske-leton fluorescence staining.The results show that CHCS can promote the cell＇s adhesion,stretched and proliferation.The introduction of cholesterol can promote the ability of chitosan as biomaterials.