中文摘要：

目的：构建杜氏盐藻叶绿体转化载体pUC-chlN1622-氯霉素乙酰转移酶（CAT）。方法：以光非依赖性的原叶绿素酸酯还原酶chlN基因为同源片段,以CAT抗性基因为选择标记,构建盐藻叶绿体转化载体pUC-chlN1622-CAT,并通过电击法转入野生型盐藻细胞,筛选转化藻株。结果：在氯霉素浓度为300mg/L的选择压力下,野生型盐藻10d左右死亡,转化藻仍正常生长,得到表达氯霉素抗性的盐藻转化株。结论：以chlN基因作为同源片段构建盐藻叶绿体转化载体是可行的。

英文摘要：

Aim：To construct the transformation vector pUC-chlN 1622-chloramphenicol acetyltransferase（CAT） for the chloroplast of Dunaliella salina（D.salina）.Methods：By using CAT gene as selective marker,and the chlN,one of light-independent protochlorophyllide reductase genes,being used as homologous segment,a transformation vector pUC-chlN 1622-CAT for the chloroplast of D.salina was constructed,which was transferred into the cells of normal D.salina,using the electroporation.Results：The transformants of D.salina survived under the selection pressure of chloramphenicol at 300 mg/L,while the wild type cells died at about 10 days after electroporation.The transformants had resistance to chloramphenicol.Conclusion：It is available to construct transformation vector for D.salina chloroplast using the chlN gene as homologous segment.