中文摘要：

运用＂通体结香＂技术诱导处理10年生白木香,以处理15 d结香后的茎干白木层（H）、过渡层及结香层（A＋T）为材料,对艾德莱EASY-spin plus RN38试剂盒RNA提取过程进行改良,并对比分析改良前后2种方法提取RNA质量的差异。结果表明：改良法提取的总RNA质量、纯度和完整性等均较RN38试剂盒好,其中28S和18S条带清晰无杂质污染,无降解,A260/A280接近2.0,RIN值大于6。说明改良后的方法适用于多年生白木香不同结香部位总RNA的提取,能满足转录组数据分析。

英文摘要：

A ten-year-old plant of agarwood（ Aquilaria sinensis） was induced by liquid transfusion technology,using healthy layer,transition and agarwood layers of the stems transfusion treated for 15 days as materials. Aidlab EASY-spin plus RN38 RNA extraction kits was modified in many key steps to improve the total RNA product qualities which were analyzed and then compared with those of the unmodified RNA extraction method. The results showed that the total RNA extracted by the modified RNA extraction method had better quality,purity and integrity than that of the unmodified method,in which the 28 S and 18 S bands were more legible with no impurity nor degradation,A260/ A280 was approximated to 2. 0,and the RIN greater than 6. This suggests that the modified method for extracting total RNA in different A. sinensis tissues transfusion treated produces high quality total RNA which can be used for transcriptome data analysis.