中文摘要：

【目的】制备与鉴定针对两种炭疽毒素受体（anthrax toxin receptor，ATR）的特异性单克隆抗体，为建立ATR的检测方法、研究炭疽毒素的致病机理提供工具。【方法】以重组表达的两种ATR即毛细血管形态发生蛋白2（capkllary morphogenesis protein 2，CMG2）和肿瘤内皮标志8蛋白（tumor endothelial marker8，TEM8）分别免疫小鼠，制备单克隆抗体，用ELISA、Western dot blotting及细胞保护性试验等方法对所制备的单克隆抗体进行鉴定和分析，并尝试应用上述单克隆抗体经双抗体夹心ELISA、Western blotting和IFA对ATR进行检测。【结果】获得针对CMG2的单克隆抗体485、4G3； 针对TEM8的单克隆抗体2D6、489；以及可同时结合CMG2和TEM8的单克隆抗体2G4，其中485具有一定的细胞保护活性，这些单克隆抗体可用于ATR的特异性检测。【结论】本研究所制备的单克隆抗体可经ELISA、Western blotting和IFA用于ATR体内外检测，从而为探讨炭疽毒素的致病机理提供有效的研究手段。

英文摘要：

[Objective] To prepare and characterize monoclonal antibodies against two types of anthrax toxin receptors （ATR） for the develop ment of rapid assays for detecting ATR and provide tools for studying the pathogenesis of anthrax toxin. [Methods] BALB/c mice were separately immunized with recombinant capillary morphogenesis protein 2 （CMG2） and tumor endothelial marker 8 （TEM8） respectively. After the fusion of mouse splenic cells with SP2/0 cells, hybridoma cell lines secreting monoclonal antibodies （McAbs） against CMG2 and TEM8 were screened by ELISA. The McAbs ob- tained were characterized by ELISA, Western dot blotting and cell protective assay, and were used in sandwich ELISA, Western blotting and IFA to detect ATRs. [Results] Five hybridoma cell lines secreting McAbs against CMG2 and TEM8 were obtained, named as 4B5, 4G3, 2G4, 2D6 and 4B9 respectively. 4B5 and 4G3 were specifically against CMG2, 2D6 and 4B9 were specifically against TEM8, 2G4 could bind both CMG2 and TEM8. Only 4B5 exhibited the cell protective activity against anthrax lethal toxin. We detected CMG2 and/or TEM8 in sandwich ELISA and Western blotting by different McAbs. [Conclusions] These McAbs may be used to detect ATRs in vitro and in vivo, and provide effective tools for studying the pathogenesis of anthrax toxin.