采用漫反射和衰减全反射两种红外光谱分析何首乌及其提取物。结果表明,以丙酮为提取剂时,何首乌其提取物中,韧皮部的有效成分含量最高,木质部的有效成分含量最低;通过两种方式的比较,发现何首乌及提取物的红外谱图存在一定的差异,何首乌在3 576和3 147cm-1有两个平缓的吸收峰,而提取物则在3 351cm-1有一强吸收峰,表明提取物中的OH…活性成分较多;同时,何首乌在931,859,766和709cm-1处有明显的吸收,而其提取物则没有,这也表明提取的是活性成分。
The diffuse-reflectance FTIR spectroscopy(DRIFTS) and attenuated total reflection FTIR(ATR-FTIR) were used to study polygonum multiflorum Thumb and its extracts.The result shows that when acetone is used as extraction agent,the contents of extracts in polygonum multiflorum Thunb's phloem are highest,those in polygonum multiflorum Thunb's xylem are the lowest.Compared with DRIFTS and ATR-FTIR,it can be found that there are some differences between polygonum multiflorum Thunb and its extracts.There are two gentle absorption peaks at 3 576 and 3 147 cm-1 respectively for polygonum multiflorum Thunb,while there is a strong absorption peak at 3 351 cm-1 for its extracts,showing that there may be more OH… active ingredients in polygonum multiflorum Thunb's extracts.Meanwhile,polygonum multiflorum Thunb has strong absorption peaks at 931,859,766 and 709 cm-1 respectively,while its extracts have no resembling absorption peaks.It also shows that the extracts are active ingredients.