中文摘要：

褐飞虱Nilaparvatalugens（StM）是一种危害水稻的重要害虫，在取食水稻时其唾液腺分泌的一些物质能激发水稻产生一系列生理生化反应。为了从褐飞虱唾液腺中得到编码这些分泌物的基因，本研究运用抑制差减杂交法（suppressedsubtractivehybridization，SSH）和镜像选择（mirrororientationselection，MOS）法，分别以取食抗虫水稻B5和敏感水稻TNl的褐飞虱唾液腺cDNA为tester和driver，构建了一个含有768个克隆子的抑制差减杂交文库。经筛选得到102个EST，插入序列长250～1000bp，代表35个单基因。其中28个表达上调，7个表达下调。经GenBank里的blastx在线分析工具分析，除了约1／3的转录序列没有相匹配的蛋白质外，其他EST所代表的氨基酸序列与已知蛋白存在不同程度的相似度，如海藻溏酶、卵黄蛋白原、ca。’结合蛋白、组织蛋白酶B（cathepsinB）、黏液样蛋白（putativemucin—likeprotein）、羧酸酯酶（carboxylesterase）和碳酸酐酶（cah·3carbonicanhydrase）等，且多数蛋白含有信号肽，推测与分泌有关。本研究将为进一步研究刺吸式昆虫中的激发子蛋白奠定了一定的基础。

英文摘要：

The brown planthopper （BPH）, Nilaparvata lugens （Stgd）, is an important rice damaging pest. Secretions by salivary glands of the BPH in the process of feeding rice contain some substances that can elicit a series of physiological and biochemical reactions of rice plants. In order to obtain the genes that encode such secretions, a suppressed subtractive hybridization （SSH） library including 768 clones was constructed by SSH and mirror orientation selection （MOS） methods with the BPH fed on the insect- resistant rice B5 as the tester and that fed on the insect-susceptible rice TN1 as the driver. The SSH library contained a total of 102 ESTs with insertions of 250 - 1 000 bp including 35 unigenes, among which 28 were up-regulated while 7 down-regulated. Through analysis by online tool blastx in the GenBank database, we found that the amino acid sequences represented by 2/3 of ESTs showed similarities in different degrees with some known proteins, such as trehalase, vitellogenin, calcium ion binding protein, cathepsin B, putative mucin-like protein, carboxylesterase, and cah-3 carbonic anhydrase. Most of the predicted proteins had signal peptides that might be related to secretion. This study laid a foundation for further research of elicitor proteins of this piercing-sucking insect.