中文摘要：

目的：研究人乳头瘤病毒（HPV）6b型L1蛋白形成的病毒样颗粒（VLPs）对女性生殖道复发性尖锐湿疣（CA）的免疫治疗作用。方法：采用HPV6bL1VLPs1μg、5μg、10μg3组剂量经0w、4w、8w免疫18例CA复发患者。分别用ELISA法和迟发性超敏反应（DTH）检测血清特异性抗体和细胞免疫效应,同时每周随访观察CA病变情况直到20w。结果：所有研究对象均能在免疫后的血清中检测到特异性抗体。各剂量组免疫前后比较,血清抗体均值间差异均有统计学意义（均P〈0.05）;所有患者DTH试验均呈阳性反应。经免疫治疗后20w的观察,77.8%（14/18）的患者CA完全消退,61.1%（11/18）的患者CA于9w之内完全消退,14例CA完全消退对象随访1年未见复发。生殖道CA消退与机体DTH反应有相关性（rs=-0.746,P=0.000）。结论：HPV6bL1VLPs可用于生殖道复发性CA的免疫治疗研究。

英文摘要：

Objective： To study the immune therapy of HPV6b virus like particles （VLPs） for female recurrent condyloma acuminatum（CA）. Methods： Eighteen studied subjects of recurrent CA were divided into three groups, to whom 1, 5, 10 μg doses of HPV6b L1 VLPs were respectively given at weeks 0, 4, and 8. The serum specific antibody （Ab） to HPV6b L1 VLPs in different therapeutic stages was detected with ELISA, and the specific cellular immunity was detected with DTH test for all subjects. All subjects were followed up once a week till the 20th week. Results： The specific serum antibody was produced in all subjects after immunization. There was a significant difference of serum Ab mean value between pre-immunization and post-immunization in three doses groups after immunization（P〈0.05）; The DTH developed in all subjects. Complete regression of CA was observed in 77.8（%14/18）subjects over 20 weeks’ observation period, and the 14 subjects didn＇t recurrent during one year after their CA regressed. The regression of CA was related to DTH development（rs=-0.746, P=0.000）. Conclusion： HPV6b L1 VLPs can be used as immunotherapy for recurrent condyloma acuminatum.