中文摘要：

目的：了解温州地方株HPV16 L1基因结构特点,分析其结构蛋白L1基因多态性及蛋白特性变化,为基于L1基因工程疫苗的设计和应用提供理论依据。方法：设计HPV16型L1基因特异性引物,从宫颈癌组织标本提取DNA,进行PCR扩增鉴定,PCR产物直接测序;进一步用Vector NTI 8.0和DNAStar软件分析HPV16型L1基因多态性及蛋白特性变化情况。结果：测定的序列与标准基因序列（NC 001526）比较,温州地区宫颈癌感染的HPV16型L1基因同源性为99.1%～99.8%,分别形成8种突变模式,其中3处由于核苷酸的改变,翻译的氨基酸也相应发生变化,但其编码蛋白在亲水性、表面可及性及抗原性上与标准株相比较无明显变化。结论：温州地方株HPV16 L1核酸有一定程度的变异。

英文摘要：

Objective：To study the character of HPV L1 gene from Wenzhou area,and analyze changes of its polymorphism and protein property.Methods：The tissue DNA was extracted from cervical carcinoma.The L1 gene of HPV was amplified with PCR by 16 type specific primers,respectively.And L1 PCR products were identified by sequencing.At last,L1 gene polymorphism and protein property were analyzed with bio-soft of Vector NTI 8.0 and DNAStar.Results： Compared with standard L1 gene sequence（NC 001526）,gene homology was 99.1%-99.8% and eight mutations were found in HPVI6 type,three of these mutations produced protein variations because of amino acids changed, but differences of their proteins were not found in hydrophilicity. surface probability and antigenic index compared with the standard L1 protein property. Conclusion： The results show that some mutation had taken place in the nucleotide sequence of L 1 gene of HPV 16 obtained from the cervical carcinoma tissues in Wenzhou area.