对SMARTTM技术构建的疣粒野生稻叶片cDNA文库克隆进行随机测序,获得了疣粒野生稻金属硫蛋白基因的cDNA序列。该序列全长412bp,开放阅读框长186bp,编码62个氨基酸,10个半胱氨酸集中分布在肽链的N端和c端,该蛋白的分子量为6.4kD,理论等电点(pI)为5.14。Blastp同源性分析表明其属于金属硫蛋白基因家族。
A metallothionein gene was cloned from Oryza meyeriana cDNA library constructed by SMART^TM technology. The eDNA was 412 bp and had an open reading frame(ORF) of 186 bp,which contained a dedueed amino acid sequence of 62 residues, 10 eysteines of the amino acid sequence distributed at protein N terminal and C terminal. The protein molecular weight is 6.4 kD and its isoelectric point is 5.14. The result of Blastp revealed that the gene is a member of metallothinein gene family.