中文摘要：

目的探讨小鼠肝细胞中表皮生长因子(epidermal growth factor,EGF)信号转导相关磷蛋白的动力学改变及其对信号转导最终生理效应的影响。方法采用双向电泳及放射性同位素标记技术,并结合相关数据库资源,分析小鼠肝细胞在饱和浓度EGF持续刺激不同时间得到的EGF信号相关磷蛋白磷酸化水平的动力学变化。结果小鼠肝细胞内EGF信号转导途径中,大多数的磷蛋白都表现为激活后很快恢复到未刺激状态;个别磷蛋白则表现为持续活化状态,如Eps15和ERK2。结论在细胞整体水平上,EGF信号途径中磷蛋白表现的动力学行为是受到整个信号转导系统整合后的动力学行为;个别磷蛋白表现为持续活化状态,这些改变可能与肿瘤的发生、发展有密切关系。

英文摘要：

Objective To study the change of dynamics of phosphorylated proteins involved in the signaling transduction of EGF in mice hepatocytes and its possible impact on ultimate physiological effects.Methods Two-dimensional electrophoresis(2-DE) method in combination with 32P isotope labeling strategy was applied to assay the dynamic behaviors of phosphoproteomes related to EGF signaling transduction in the whole hepatocytes.ResultsThe dynamic behaviors of most of phosphorylated proteins had been presented in a pe...