试验研究了半胱胺(Cs)对藏绵羊断奶羔羊瘤胃pH值、总脱氢酶及TVFA的影响。将10只(公母各半)80日龄左右、体重平均为(16.00±0.31)kg、装有永久性瘤胃瘘管的藏绵羊断奶羔羊随机分为2组(试验组和对照组),在日粮精料中添加300 mg/(kg·BW)的Cs,隔日添加1次,试验期42 d。正式试验当日及试验第7、14、21、28、35天的7:00、9:00、11:00、13:00、15:00、17:00、19:00、21:00、23:00、1:00、3:00、5:00于颈静脉采血样10 mL,同时采集瘤胃液10 mL,测定瘤胃pH值、总脱氢酶的活性和TVFA。检测结果表明,试验组精料中添加Cs使藏绵羊断奶羔羊瘤胃液pH值升高;血清尿素氮浓度降低,与对照组相比差异极显著(P〈0.01);瘤胃液总脱氢酶活力提高极其明显(P〈0.01);整个试验期内,半胱胺对瘤胃TVFA的影响极显著(P〈0.01)。
The effects of cysteamine (Cs) on pH, total dehydrogenase and TVFA in the rumen of weaned lambs of Tibetan Sheep were studied. 10 80-day-old weaned lambs of Tibetan Sheep with loading permanent rumen fistula and the average initial weight of(16.00±0.31)kg were randomly divided into two groups(test group and control group). 300 mg/(kg·BW) of Cs was added in the diet concentrate every two days. The test period lasted for 42 days. 10 mL vein blood samples and tumor gastric juice were collected at 7:00, 9:00, 11:00, 13:00, 15:00, 17:00, 19:00, 21:00, 23:00, 1:00, 3:00 and 5:00 on the 0th, 7th, 14th, 21st, 28th and 35th days. And pH, total dehydrogenase activity and TVFA in the rumen were determined. The results showed that adding Cs in the concentrate in the test group increased pH of rumen juice in the weaned lambs of Tibetan Sheep and decreased the concentration of urea nitrogen in the serum, which had significant difference with control group (P〈0.01). And the activity of total dehydrogenase in gastric juice was significantly enhanced (P〈0.01). In the whole test period, Cs had extremely significant influences on TVFA in the rumen.