利用幼畜胚胎体外生产与胚胎移植(Juvenile in vitro embryo transfer,JIVET)技术能够获得大量卵母细胞,缩短世代间隔,加快育种进程.但幼畜卵母细胞形态及代谢与成年动物存在差异,这导致幼畜胚胎发育能力低.目前,对JIVET技术的研究主要是通过改进激素处理方案及体外培养条件来增加卵母细胞数量并增强卵母细胞发育能力.从供体羔羊的选择、激素处理和卵母细胞的发育等方面对该技术进行了分析.
Juvenile in vitro embryo transfer(JIVET) method could get large number of oocytes,reduce the generation interval and increase the animal breeding programs.But the morphologic and metabolic of the oocytes in the juveniles were different from the mature livestocks which led to the low development ability of juvenile embryo in vitro.The main study of JIVET in recent years were to increase the oocytes number and developmental ability through improving hormone stimulation regime and in vitro culture conditions.This review included the analysis on every respect of JIVET in lamb such as donor selection,hormone stimulation and oocytes development.